25.00.35.Геоинформатика

Clinical and epidemiological characteristics of rotavirus infection  infants This paper deals with epidemiological analysis of rotavirus gastroenteritis in infants in the first year of life and the study of nosocomial spread of diseases among them in the hospital. Shown widespread rotavirus gastroenteritis among children first month of life, the possibility of a wide nosocomial rotavirus infection children. The results obtained allowed for more effective prevention and control measures in the foci of infection.

Alexandr Fedorovich Lebedev —  first soil scientist Rostov University The article describes the main stages of the life and work of an outstanding representative of Russian genetic soil science Alexandr Fedorovich Lebedev. The authors attempted to collect anecdotal information about the first soil scientist Rostov University, who worked within its walls long before the organization of the Department of soil science.

Ecological State of Dark Chestnut Soil in Agrogene-transformed Ecosystems of Orenburg Zauralye The dark chestnut soil in various farmland are investigated in Orenburg Zauralye. The comparison of soil with virgin analogues showed that the degree of agrogenic transformation and environmental degradation of these soils increases in the following series: virgin — hayfield — grazing areas — fallow land — arable land.

Identification of fish species of the family of whitefish, harvested on the North Yenisei With the implementation of raw fish in the settlements of the Yenisei North revealed falsification of fish whitefish family, which is expressed in a specific regrading. During the study, the analysis of the scheme regrading Arctic cisco, Siberian whitefish and muksun produced in the lower reaches of the river basin Yenisei. Proposed a rapid method of identification based on knowledge of the morphometric characters.

Features reovirus RNA isolation from clinical material S. A. Kolpakov, E. P. Kolpakova. Key objective of the given research is to find out reasons of failures during extraction of the RNK reoviruse from sick people clinical material. In due course it was discovered that transcriptase (RNA dependent RNA polymerase) very quickly transforms double-stranded RNA into a single-stranded RNA due to short time of activation after violation of reoviruse outside capsid integrity. This single-stranded RNA is invisible using standard way of electrophoresis registration. Addition of a transcriptase with polymerase function in ready-made samples or in clinical material from sick people before RNA extraction leads to transformation of a single-stranded RNA (if available) into a dsRNA identified after electrophoresis in the form of genome’s segments. The developed RNA visualisation method could assist in etiological diagnostics of reoviral infection and in studying of reovirus as a pathogen.

New group of human rotaviruses, the family Reoviridae S. A. Kolpakov, E. P. Kolpakova. Key objective of the given research is to find out reasons of failures during extraction of the RNK reoviruse from sick people clinical material. In due course it was discovered that transcriptase (RNA dependent RNA polymerase) very quickly transforms double-stranded RNA into a single-stranded RNA due to short time of activation after violation of reoviruse outside capsid integrity. This single-stranded RNA is invisible using standard way of electrophoresis registration. Addition of a transcriptase with polymerase function in ready-made samples or in clinical material from sick people before RNA extraction leads to transformation of a single-stranded RNA (if available) into a dsRNA identified after electrophoresis in the form of genome’s segments. The developed RNA visualisation method could assist in etiological diagnostics of reoviral infection and in studying of reovirus as a pathogen.

Microbiocenosis of cavity uteri in missed abortion Studied complex microbiological and morphological study of endometrial scrapings 244 women with missed abortion. In the study found the prevalence of mixed viral and bacterial infections. Frequent combination of viruses marked with mycoplasma, ureaplasma and chlamydia.

Viral safety of blood products Grehirchak Nataliya Nykolayvna, Lych Inna Valentynovna The article deals with issue of ensuring viral safety of blood products. We defined responsibility and rightness of using method of polymerase chain reaction during production of blood specimen, appraised the quality of products for their promotion. The definition of contamination materials (plasma and precipitations), intermediate metabolite was carried out by method of PRC on the content of nucleic acid parvovirus B19, hepatitis B virus, hepatitis C virus in order to confirm the possibility of using abovementioned methods for quality assurance of blood components.

Problems and challenges of soil science application to developing information technologies in agricultural production of Rostov region Golozubov Oleg Modestovich, Kol’chik Anatolij Fyodorovich, Kryshhenko Vladimir Stefanovich, Litvinov Yurij Alekseevich The achievements, problems, and challenges of the modern stage of the development of the GIS for agromonitoring of agricultural lands in Rostov oblast. The structure of the information system of the GIS as an internet-based resource and its application for solving practical problems are described. For the first time in Russia, the GeoRSS standard based on the structured hypertext representation of the geographic and attribute information has been applied in the state system for the agromonitoring of agricultural lands in Rostov oblast and information exchange through the internet.

Resistance of microorganisms to antimicrobial drugs Pokudina Inna Olegovna, Shkurat Mihail Alekseevich, Battalov Dmitrij Valentinovich Abstract: The emergence of the phenomenon of resistance to therapeutic drugs in the pathogenic microorganisms, has a very negative effect on the health of humanity, because it leads to a sharp decrease in the efficiency of etiotropic therapy  of infectious diseases. Intense selective pressure of antibiotics leads to the rapid evolution and spread of new resistance mechanisms in health care settings and, above all, in the Intensive care unit. The article analyzes the literature data about the mechanisms of emergence and transmission of the resistance of pathogens to antimicrobial agents and acquired resistance associated with the production of beta-lactamases extended spectrum.

Clinical and epidemiological characteristics of rotavirus infection  infants This paper deals with epidemiological analysis of rotavirus gastroenteritis in infants in the first year of life and the study of nosocomial spread of diseases among them in the hospital. Shown widespread rotavirus gastroenteritis among children first month of life, the possibility of a wide nosocomial rotavirus infection children. The results obtained allowed for more effective prevention and control measures in the foci of infection.

Alexandr Fedorovich Lebedev —  first soil scientist Rostov University The article describes the main stages of the life and work of an outstanding representative of Russian genetic soil science Alexandr Fedorovich Lebedev. The authors attempted to collect anecdotal information about the first soil scientist Rostov University, who worked within its walls long before the organization of the Department of soil science.

Ecological State of Dark Chestnut Soil in Agrogene-transformed Ecosystems of Orenburg Zauralye The dark chestnut soil in various farmland are investigated in Orenburg Zauralye. The comparison of soil with virgin analogues showed that the degree of agrogenic transformation and environmental degradation of these soils increases in the following series: virgin — hayfield — grazing areas — fallow land — arable land.

Identification of fish species of the family of whitefish, harvested on the North Yenisei With the implementation of raw fish in the settlements of the Yenisei North revealed falsification of fish whitefish family, which is expressed in a specific regrading. During the study, the analysis of the scheme regrading Arctic cisco, Siberian whitefish and muksun produced in the lower reaches of the river basin Yenisei. Proposed a rapid method of identification based on knowledge of the morphometric characters.

Features reovirus RNA isolation from clinical material S. A. Kolpakov, E. P. Kolpakova. Key objective of the given research is to find out reasons of failures during extraction of the RNK reoviruse from sick people clinical material. In due course it was discovered that transcriptase (RNA dependent RNA polymerase) very quickly transforms double-stranded RNA into a single-stranded RNA due to short time of activation after violation of reoviruse outside capsid integrity. This single-stranded RNA is invisible using standard way of electrophoresis registration. Addition of a transcriptase with polymerase function in ready-made samples or in clinical material from sick people before RNA extraction leads to transformation of a single-stranded RNA (if available) into a dsRNA identified after electrophoresis in the form of genome’s segments. The developed RNA visualisation method could assist in etiological diagnostics of reoviral infection and in studying of reovirus as a pathogen.

New group of human rotaviruses, the family Reoviridae S. A. Kolpakov, E. P. Kolpakova. Key objective of the given research is to find out reasons of failures during extraction of the RNK reoviruse from sick people clinical material. In due course it was discovered that transcriptase (RNA dependent RNA polymerase) very quickly transforms double-stranded RNA into a single-stranded RNA due to short time of activation after violation of reoviruse outside capsid integrity. This single-stranded RNA is invisible using standard way of electrophoresis registration. Addition of a transcriptase with polymerase function in ready-made samples or in clinical material from sick people before RNA extraction leads to transformation of a single-stranded RNA (if available) into a dsRNA identified after electrophoresis in the form of genome’s segments. The developed RNA visualisation method could assist in etiological diagnostics of reoviral infection and in studying of reovirus as a pathogen.

Microbiocenosis of cavity uteri in missed abortion Studied complex microbiological and morphological study of endometrial scrapings 244 women with missed abortion. In the study found the prevalence of mixed viral and bacterial infections. Frequent combination of viruses marked with mycoplasma, ureaplasma and chlamydia.

Viral safety of blood products Grehirchak Nataliya Nykolayvna, Lych Inna Valentynovna The article deals with issue of ensuring viral safety of blood products. We defined responsibility and rightness of using method of polymerase chain reaction during production of blood specimen, appraised the quality of products for their promotion. The definition of contamination materials (plasma and precipitations), intermediate metabolite was carried out by method of PRC on the content of nucleic acid parvovirus B19, hepatitis B virus, hepatitis C virus in order to confirm the possibility of using abovementioned methods for quality assurance of blood components.

Problems and challenges of soil science application to developing information technologies in agricultural production of Rostov region Golozubov Oleg Modestovich, Kol’chik Anatolij Fyodorovich, Kryshhenko Vladimir Stefanovich, Litvinov Yurij Alekseevich The achievements, problems, and challenges of the modern stage of the development of the GIS for agromonitoring of agricultural lands in Rostov oblast. The structure of the information system of the GIS as an internet-based resource and its application for solving practical problems are described. For the first time in Russia, the GeoRSS standard based on the structured hypertext representation of the geographic and attribute information has been applied in the state system for the agromonitoring of agricultural lands in Rostov oblast and information exchange through the internet.

Resistance of microorganisms to antimicrobial drugs Pokudina Inna Olegovna, Shkurat Mihail Alekseevich, Battalov Dmitrij Valentinovich Abstract: The emergence of the phenomenon of resistance to therapeutic drugs in the pathogenic microorganisms, has a very negative effect on the health of humanity, because it leads to a sharp decrease in the efficiency of etiotropic therapy  of infectious diseases. Intense selective pressure of antibiotics leads to the rapid evolution and spread of new resistance mechanisms in health care settings and, above all, in the Intensive care unit. The article analyzes the literature data about the mechanisms of emergence and transmission of the resistance of pathogens to antimicrobial agents and acquired resistance associated with the production of beta-lactamases extended spectrum.