03.02.14 Биологические ресурсы

Clinical and epidemiological characteristics of rotavirus infection  infants This paper deals with epidemiological analysis of rotavirus gastroenteritis in infants in the first year of life and the study of nosocomial spread of diseases among them in the hospital. Shown widespread rotavirus gastroenteritis among children first month of life, the possibility of a wide nosocomial rotavirus infection children. The results obtained allowed for more effective prevention and control measures in the foci of infection.

Alexandr Fedorovich Lebedev —  first soil scientist Rostov University The article describes the main stages of the life and work of an outstanding representative of Russian genetic soil science Alexandr Fedorovich Lebedev. The authors attempted to collect anecdotal information about the first soil scientist Rostov University, who worked within its walls long before the organization of the Department of soil science.

Ecological State of Dark Chestnut Soil in Agrogene-transformed Ecosystems of Orenburg Zauralye The dark chestnut soil in various farmland are investigated in Orenburg Zauralye. The comparison of soil with virgin analogues showed that the degree of agrogenic transformation and environmental degradation of these soils increases in the following series: virgin — hayfield — grazing areas — fallow land — arable land.

Identification of fish species of the family of whitefish, harvested on the North Yenisei With the implementation of raw fish in the settlements of the Yenisei North revealed falsification of fish whitefish family, which is expressed in a specific regrading. During the study, the analysis of the scheme regrading Arctic cisco, Siberian whitefish and muksun produced in the lower reaches of the river basin Yenisei. Proposed a rapid method of identification based on knowledge of the morphometric characters.

Features reovirus RNA isolation from clinical material S. A. Kolpakov, E. P. Kolpakova. Key objective of the given research is to find out reasons of failures during extraction of the RNK reoviruse from sick people clinical material. In due course it was discovered that transcriptase (RNA dependent RNA polymerase) very quickly transforms double-stranded RNA into a single-stranded RNA due to short time of activation after violation of reoviruse outside capsid integrity. This single-stranded RNA is invisible using standard way of electrophoresis registration. Addition of a transcriptase with polymerase function in ready-made samples or in clinical material from sick people before RNA extraction leads to transformation of a single-stranded RNA (if available) into a dsRNA identified after electrophoresis in the form of genome’s segments. The developed RNA visualisation method could assist in etiological diagnostics of reoviral infection and in studying of reovirus as a pathogen.

New group of human rotaviruses, the family Reoviridae S. A. Kolpakov, E. P. Kolpakova. Key objective of the given research is to find out reasons of failures during extraction of the RNK reoviruse from sick people clinical material. In due course it was discovered that transcriptase (RNA dependent RNA polymerase) very quickly transforms double-stranded RNA into a single-stranded RNA due to short time of activation after violation of reoviruse outside capsid integrity. This single-stranded RNA is invisible using standard way of electrophoresis registration. Addition of a transcriptase with polymerase function in ready-made samples or in clinical material from sick people before RNA extraction leads to transformation of a single-stranded RNA (if available) into a dsRNA identified after electrophoresis in the form of genome’s segments. The developed RNA visualisation method could assist in etiological diagnostics of reoviral infection and in studying of reovirus as a pathogen.

Microbiocenosis of cavity uteri in missed abortion Studied complex microbiological and morphological study of endometrial scrapings 244 women with missed abortion. In the study found the prevalence of mixed viral and bacterial infections. Frequent combination of viruses marked with mycoplasma, ureaplasma and chlamydia.

Viral safety of blood products Grehirchak Nataliya Nykolayvna, Lych Inna Valentynovna The article deals with issue of ensuring viral safety of blood products. We defined responsibility and rightness of using method of polymerase chain reaction during production of blood specimen, appraised the quality of products for their promotion. The definition of contamination materials (plasma and precipitations), intermediate metabolite was carried out by method of PRC on the content of nucleic acid parvovirus B19, hepatitis B virus, hepatitis C virus in order to confirm the possibility of using abovementioned methods for quality assurance of blood components.

Problems and challenges of soil science application to developing information technologies in agricultural production of Rostov region Golozubov Oleg Modestovich, Kol’chik Anatolij Fyodorovich, Kryshhenko Vladimir Stefanovich, Litvinov Yurij Alekseevich The achievements, problems, and challenges of the modern stage of the development of the GIS for agromonitoring of agricultural lands in Rostov oblast. The structure of the information system of the GIS as an internet-based resource and its application for solving practical problems are described. For the first time in Russia, the GeoRSS standard based on the structured hypertext representation of the geographic and attribute information has been applied in the state system for the agromonitoring of agricultural lands in Rostov oblast and information exchange through the internet.

Resistance of microorganisms to antimicrobial drugs Pokudina Inna Olegovna, Shkurat Mihail Alekseevich, Battalov Dmitrij Valentinovich Abstract: The emergence of the phenomenon of resistance to therapeutic drugs in the pathogenic microorganisms, has a very negative effect on the health of humanity, because it leads to a sharp decrease in the efficiency of etiotropic therapy  of infectious diseases. Intense selective pressure of antibiotics leads to the rapid evolution and spread of new resistance mechanisms in health care settings and, above all, in the Intensive care unit. The article analyzes the literature data about the mechanisms of emergence and transmission of the resistance of pathogens to antimicrobial agents and acquired resistance associated with the production of beta-lactamases extended spectrum.

Total ecosystem respiration and evapotranspiration of steppe catena (West Siberia) The article deals with the diurnal dynamics of CO2 emission and evapotranspiration of steppe ecosystems. The profiles of carbon dioxide distribution in soil atmosphere were set under soil salinity conditions. The findings help to explain the ecological function of steppe catena.

Features of action of weak low-frequency alternating magnetic field on the intensity of division planarian Dugesia tigrina at static magnetic field with "zero" and geomagnetic values It have been shown that exposure of the planarians to low-frequency alternating magnetic field (frequency of 3,7 Hz, the amplitude of 40 nT, exposure 4 h) in combination with a static magnetic field collinear with the induction of 42 mT promoted its division. On other hand compensation of the static magnetic field (induction £ 0,1 mT) by means of alternating magnetic field resulted in the opposite directional inhibitory effect consisted of reduction of the number of divisions of planarians.

Dynamics of toxicity waters of the small river within megapolis (r. Temernik, SFD) Ecological problems of small rivers were considered. Based on years of material toxicity waters r. Temernik (SFD) at bioassay was shown the evolution of toxicity classes and ecological status of water.

Erosion processes on the territory of small catchment — regularities of formation Analysis of the results four years of research of the snowmelt runoff formation and soil loss on the catchment area of the small river Lubozikha showed that surface runoff and soil washout has an influence not only on the intensity of erosion processes, but also on the chemical composition of river waters.

To the study of water mites (Acariformes) as part of a small river Devitsa Zooperiphiton (Voronezh region) An ecologo-faunistic information about 13 species from 6 families of water mites of zooperiphyton  river Devitsa (Limestone North Central Russian upland, Voronezh region), of which 10 are listed for the first time for the region. Identified dominant and rare species, preferences in of various substrates.

Effects of partial cutting on soil microbial complex in pinewoods of Krasnoyarsk forest-steppe region The analysis of a structural-functional condition of soil microbita has shown, that satisfactory soil forest-vegetation conditions the pinewoods density of 0,6—0,7 which have been passed by the first cutting. Intensity of felling a forest stand, the season of lumbering and a way of brush disposal the promote redistribution in structure of eco-trophic groups of microorganisms and formation of an optimum level of enzymes activity in soil.

The role of agrochemistry in the conditions of modern agriculture in Russia An analysis of current problems and the negative consequences of underestimating major laws of agriculture in the Russia were carried out. Have considered the ecological function of agrochemistry in agroecosystems and the conditions which necessary for the successful development and modernization of  agriculture of the country.

The soil and microbiological characteristics of virgin and deposit gray forest soils of Priangarye, are formed in conditions of hillocky relief One of the factors that determine the characteristics of soil formation and soil microbial processes in the Priangarye is hillocky relief. It promotes differentiation of soil-forming processes in the soil profile and a sharp difference of their morphology and properties. When plowing of soil is a significant change in the properties of soils [12] and microbiological parameters [13], which for a long time is saved when translated into deposit mode.

Introduction of trees and shrubs in the Nikita Botanical Gardens and their naturalization on the Crimean peninsula The check-list of trees and shrubs presented in collection of the Nikita Botanical Gardens capable for self-reproduction or run wild on the territory of Crimean peninsula have been provided. Invasive activity of species have been given according to the categories used in the database on naturalized species in European botanical gardens.

Agroecological efficiency of use of rice meliorative cultivated lands on the basis of the evaluation of agricultural resource territory potential The approbation of methodological approaches according to the agroecological evaluation of lands of rice irrigation system has been conducted. Based on the analysis of statistic and predicted data on yield capacity of rice crop rotation it has been proved that it can be eligible for developing farming system in rice cultivation. The correspondence between actual and calculated according to the degree method of agroecological land quality has been discovered.