03.02.02 Вирусология

Transformation of soils in urbanized landscapes of khabarovsk under the influence of hydrological processes         Roslikova Valentina Ivanovna          Absract. The transformation of the natural environment, including the soil mantle, in the Far Eastern landscapes occurs under the influence of various types of modern geological processes, conditioned not only by the specifics of natural factors, but by a whole series of man-made ones. The last ones lead to the activation of geological processes and the loss of not only the stability of structures, but also to a shift in the state of the soil mantle. This issue has not been investigated for the soils of the Far Eastern urban landscapes. To reveal the morphodiagnostic transformation of soils in urban areas, it is necessary to disclose the patterns of their transformation under the influence of dominant processes. These are technogenic hydrogeological processes that cause flooding and underflooding. The study is based on a new all-Russian classification of soils.       The objects of the study were two catenas located in different engineering and geological regions of a large industrial center in Khabarovsk with various types of technogenic hydrogeological processes. Diagnostic signs of transformed soils and new predpochvennye formations have been determined by means of the elementary landscapes of various sections of the catena, and the mechanisms of these transformations have been revealed. In the zone of technogenic flooding the relationship between the diagnostic features of transformed soils and the new environmental conditions is analyzed. Against the backdrop of landslide processes in the zone of technogenic flooding the nature of the soils transformation is considered. They were investigated in two blocks: man-made and mineral. The structure and mechanisms of creep in these blocks are different, which is clearly documented in the nature of the sliding material and the gradualness of the soil mantle transformation. The allocation of a new predpochvennye formation - fractal-stratified - is justified.

On the biological degradation of white phosphorus On the biological degradation of white phosphorus   1 Institute of Organic and Physical Chemistry named after A.E. Arbuzov. Kazan Scientific Center of the Russian Academy of Sciences. Arbuzov St., 8. Kazan, 420088. Republic of Tatarstan. Russia. E-mail: mindubaev-az@yandex.ru 2 Kazan (Volga Region) Federal University. University St., 18. Kazan, 420008. Republic of Tatarstan. Russia. Abstract For the first time different taxonomic groups of microorganisms (fungi, Streptomyces and bacteria) are inoculated on culture medium containing white phosphorus as the single source of phosphorus. On these media microorganisms grew and have not experienced phosphorus starvation. It is the world's first example of the inclusion of white phosphorus in the biosphere cycle of elemental phosphorus. The highest concentration corresponds to 5000 times excess of MPC of white phosphorus in wastewater. The comparison of the sequences of ribosomal genes of the fungus, steadily metabolizing the white phosphorus, with sequences of the GenBank database, allowed us to identify this microorganism as a new strain of Aspergillus niger, to which we have assigned the number A. niger AM1. Inoculation of A. niger AM1 in medium containing just two sources of phosphorus (phosphate and white phosphorus) demonstrated that P4 does not exhibit toxic properties in relation to this microorganism. In the presence of white phosphorus it grows at the same rate as in the absence thereof. This is the only example of the lack of white phosphorus toxicity to a living organism. Our previous studies have demonstrated the absence of white phosphorus toxicity for Aspergillus niger AM1. However, the toxic properties of the substances are of different nature. It is of great interest to study the genotoxicity – a possible source of mutations. In the present work SOS-lux test has demonstrated genotoxicity of white phosphorus. This result is obtained for the first time – all the available literature sources reported no genotoxic properties of white phosphorus. Allium test showed the mitotoxic effect of white phosphorus on eukaryotic cells. A very interesting and unexpected result was the discovery of a mutant Aspergillus niger AM1. In comparison with an ancestral strain this mutant possesses an unusual morphology and grows more intensively in culture medium with white phosphorus. Three strains of A. niger, sent from the All-Russian collection of microorganisms, also showed much higher resistance to white phosphorus than bacteria. According to preliminary data, resistance to white phosphorus the A. niger is fixed in the genome.

Resource-saving elements of soybean cultivation technology on the slopes of the Rostov region Resource-saving elements of soybean cultivation technology on the slopes of the Rostov region Rychkova M.I., Gaeva E.A., Mishchenko A.V. Federal Rostov Agricultural Research Center, Rassvet. Е-mail: emmaksay@inbox.ru. Abstract. The article presents the actual results of research on the development of resource-saving elements of soybean cultivation technology, providing moisture saving, high productivity and soil protection from erosion. Two-year studies were carried out on the erosion-dangerous slope of the South-Eastern exposure of the Bolshoy Log beam, Aksai district of the Rostov region in the system of contour-landscape organization of the territory in 2017-2018. Studied two methods of primary tillage – otvalnuyu adopted for monitoring and chisel. Chisel tillage in the cultivation of soybean was performed by chisel plow. Dump processing was carried out with a plow blade. The mineral nutrition system included three backgrounds: natural fertility recommended by zonal systems and increased by half. It is established that to prevent possible erosion it is recommended to cultivate soybeans in the system of contour-band placement of fields. It is shown that the payback of the crop fertilizers on the version with the chisel treatment of the soil is higher than on the dump. Increasing the dose of fertilizer in half reduces the payback of fertilizers by 18.9-19.4 %. It is revealed that the approved soil protection (chisel) tillage can significantly reduce the erosion processes – soil flushing by 22.9%, runoff – 10.0%, the cost of fuel and lubricants-18.8%, increase the accumulation of reserves of productive moisture in the soil with a more economical expenditure, yield – by 30.2 – 32.1%, conditional net income-up to 6.6 thousand rubles /ha, profitability-by 42.1%, recoupment of direct costs yield of 1.4 rubles per ruble in comparison with the same indicators at the dump plowing.

Location on the Caucasus Potentilla multi-advisable (Potentilla multifida L.) Location on the Caucasus Potentilla multi-advisable (Potentilla multifida L.)  O.N. Demina, L.L. Rogal'  Abstract: The paper describes the Gum-Bashi Pass, which is named after the homonymous right-bank tributary of the Kuban - the Mara River. The distribution of grass vegetation on the Gum-Bashi Pass, as on the whole Rocky Caucasus Mountains, is associated with vertical zonality or altitudinal belt ecosystems. The characteristic of indigenous subalpine vegetation is given: on gentle northern slopes and plateaus of the Rocky ridge formed lush grassy meadows; Petrophytic steppes develop on the steep slopes of the southern, southeastern, and southwestern exposures. The most common types of natural forage lands on the Gum-Bashi Pass are mountain meadow communities on mountain sod-podzolic, gray and brown forest soils, and meadow-steppe communities on leached and podzolized black soil. However, the main purpose of the work is to describe a new plant species, Potentilla multifida L., which we discovered for the first time in the territory of Karachay-Cherkessia, in the Rocky Ridge system on the Gum-Bashi Pass and confirming its growth in the Caucasus. In describing this species, it is mentioned that this herbaceous perennial reaches 40 cm in height. The leaves of the plant are green above and beloiclochic below, dissected into linear lobes. The flowers are small, on thin pedicels, pale yellow. The beginning of its flowering was recorded when the species was collected. A critical summary of references to this species and an indication of the need to describe a new Caucasian form is given. In conclusion, one more time focuses on the need for this description, studying the distribution of P. multifida in the Caucasus and its phytocenotic environment. The article is illustrated with a large number of photos.

Effect of different phytohormones on Austrian sage multiplication (Salvia austriaca Jacq.) Effect of different phytohormones on Austrian sage multiplication (Salvia austriaca Jacq.) Vasilchenko Elena V., Sereda Mikhail M., Varduni Tatiana V. Southern Federal University, Rostov-on-Don, Russia  Abstract: The influence of phytohormones such as 6-benzylaminopurine, naphthylacetic acid and indole-3-acetic acid on the Austrian sage multiplication (Salvia austriaca Jacq.) in an in vitro culture is discussed in the paper. Austrian sage is the medicinal, essential oil, melliferous and ornamental steppe herb, included in the Red List of the Rostov region. The study was conducted in the laboratory of cellular and genomic technologies of plants of the Botanical Garden. Seeds from the collection of the Botanical Garden of the Southern Federal University were used as the primary explant. For cultivation the Murashige and Skoog medium was used. Murashige and Skoog medium without the addition of phytohormones was used as a control. Evaluation of the multiplication efficiency was carried out after 21 days. The multiplication factor was calculated as the number of new shoots. The highest multiplication factor was observed in the medium with the addition of 0.5 mg / l of 6-benzylaminopurine and 0.1 mg / l of naphthylacetic acid. This concentration of phytohormones is recommended for micropropagation of Austrian sage. The concentration of auxins (IAA and NAA) 0.3 mg/l leads to a decrease in the efficiency of Austrian sage multiplication. The height of shoot was approximately the same and no statistically significant differences were found on all tested mediums. It should be pointed out that with a greater concentration of phytohormones physiological abnormalities was observed.

Methods of soil fertility preservation on eroded slopes of Rostov region Methods of soil fertility preservation on eroded slopes of Rostov region Gaevaia Е.А. Federal Rostov Agricultural Research Center, Rassvet. Е-mail: emmaksay@inbox.ru. Abstrac: Тhe Studies were carried out in a multifactorial stationary experiment in 1991-2018 years. the Experiment is located on the slope of the beam of the Big Log of Aksai district of Rostov region. The soil of the experimental site is ordinary heavy loamy Chernozem on loess-like loam, medium-eroded. In the experiment studied two crop rotation zernopererabotki (I) and sertralineprograms (II), and three levels of organic-mineral system of fertilizers («0» – natural fertility; «1» – cattle manure 5 t + N46P24K30 and «2» – cattle manure 8 t + N84P30K48 1 hectare). The aim of the study was to study the balance of humus depending on the design of crop rotations located on the eroded slopes of the Rostov region, to identify ways to preserve soil fertility at the initial level and increase it. As a result of the research, the amount of washed soil was determined. The importance of plant residues in the accumulation of humus is shown. It is established that the intensive use of agricultural land in the Rostov region without fertilizer leads to a reduction in the humus content in the soil. The role of perennial grasses in reducing soil flushing by 35.6% was studied. It is shown that the long-term use of organic fertilizers in crop rotations at a dose of 5 tons of cattle manure and N46P24K30, as well as the introduction of 20% of perennial in the crop rotation allows to maintain a positive balance of humus at the level of (+35 kg/ha) in the grain-grass crop rotation. Increasing the dose of fertilizer to 8 tons of cattle manure and N84P30K48 contributes to the accumulation of humus up to (+100 kg/ha) in the grain crop rotation. The possibility of replacing organic fertilizers with 1-2 t/ha of wheat straw using compensatory doses of nitrogen fertilizers is shown.

To the future without poliomyelitis: the state of the epidemic, vaccination issues (review) To the future without poliomyelitis: the state of the epidemic, vaccination issues (review) Belova Olga Evgen’evna  FSRI SRC VB «Vector», Koltsovo, Novosibirsk reg., Russia  Abstract: Polio is a highly contagious viral infection that affects mainly children under five years of age, which can lead to paralysis or even death. Damage to the nervous system is manifested by fever, pain in the limbs, then peripheral irreversible paralysis. In severe cases, paralysis of the respiratory muscles can occur, which causes death. The transmission mechanism is predominantly fecal-oral. The only way to combat polio remains vaccination. The vaccine provides the development of long-term humoral and local immunity. The purpose of this work is to analyze the epidemiological situation of poliomyelitis in the world 31 years after the adoption of the Global Polio Eradication Program, and to compare the characteristics of live and inactivated polio vaccines. Issues related to the use of live oral polio vaccine and the need for its phased withdrawal from circulation, the problem of vaccine-related polioviruses and vaccine-associated paralytic polio are considered. Describes ongoing research into the development of new safe and effective polio vaccines; approaches to the development of new IPV to reduce costs, increase coverage and reduce the risks of using live polioviruses in vaccine production; new technologies for the delivery and administration of IPV; clinical studies of new vaccines and vaccination schedules. The main objectives of the strategy to complete the eradication of poliomyelitis for 2019-2023 are discussed: eradication, integration, certification and containment.

The ameliorative condition of the soil spread around the Kattakurgan reservoir and its changes The ameliorative condition of the soil spread around the Kattakurgan reservoir and its changes Abdullaev S.A.1, Jabbarov Z.A.1, Tursunulova A.1, Okolelova A.A.2, Kholdorov Sh.M.3 1National University of Uzbekistan, Tashkent, Uzbekistan, zafarjonjabbarov@gmail.com 2Volgograd State Technical University, Volgograd, Russia, allaokol@mail.ru 3Tokyo University of Agriculture and Technology – TUAT, Tokyo, Japan, sh.m.xoldorov@gmail.com Abstract: Changes in the physicomechanical properties and ameliorative state of the soil were studied using the Kattakurgan reservoir as an example; the degree of fossil, the density of irrigated lands, the dynamics of the groundwater level by years, the ameliorative state and the location of salts by layers were studied. According to the results, the indicator of soils with an inadequate ameliorative state increased from 4.22% to 5.58%. It was justified that the level of groundwater salinity is mainly between 0-1 g / l and 1-3 g / l. It is scientifically substantiated that the main effect of the Kattakurgan reservoir on soils is formed by the accumulation of salts, the effect on the physicomechanical properties is relatively small.

Diagnosis and nomenclature floodplains soils in ecology-genetic and substantively-genetic classification and on the common State Register of soil resources of Russia When diagnosing and soil nomenclature River floodplain associated ecological-genetic classification, substantively-genetic classification and the unified State registry of soil resources of Russia. Studies were conducted on the ecosystem, located on the right bank of the plot of the floodplain of the middle reaches of the River Desna. The plot includes 12 floodplain environmental subsystems of different order. Results of the field studies allow, after receiving additional analytical data and conduct relevant discussions initiate a clarification on a number of issues of classification, diagnosis and nomenclature floodplains soils. At the present stage, it is advisable to use the Unified State Register of Soil Resources of Russia for soil surveys of floodplains.  

The balance of humus in crop rotations with short rotation on erosion-prone slopes Gaevaya Emma Аnatolievna State scientific institution Don Research Institute of Agriculture  Abstract: The purpose of this work is to study the balance of humus and its reproduction in crop rotations with different content in the structure of the acreage of pure fallow and perennial grasses on the slopes of the Rostov region. Studies were conducted in multifactor stationary experiment, located on the side of the beam Large Log in 2003-2017 years. Experiment was laid in 1986 in the system of contour-landscape organization of the territory of the slope with suppress of 3.5-4°. The soil of experimental plot – the ordinary heavy on loess-like loam. The initial content of humus in the soil at the laying of experiment was 3.80-3.83%. I was studied three crop rotations with different percentages of black allow and perennial grasses in the structure of crops rotations. The article presents data of the post-harvest residues amount and nitrogen entering the soil after different crops. The average values of root and crop residues catering in the soil for a hectare of crop rotation area are presented. The balance of humus is calculated when different doses of fertilizers are applying. The role of balk follow and perennial grasses in maintaining the deficit-free balance of humus was studied. It was shown that to achieve a deficit-free humus balance at the ordinary chernozems in crop rotations, located on erosion-prone slopes of the Rostov region, it is necessary to add organic fertilizer in a dose of 100 kg/ha considering the flushing of the soil. Expanded reproduction of soil fertility is possible if you add organic and mineral fertilizers in a dose of 162 kg/ha and crop rotation with 40 % of perennial grasses.

The influence of the groundwater level regime on the biological productivity of wood species in the forest bands of the Stone Steppe The influence of the groundwater level regime on the biological productivity of wood species in the forest bands of the Stone Steppe CheverdinYurii I.1, Akhtyamov Alexander G.2,Sautkina Marina Yu.3, 1V.V. Dokuchaev Scientific Research Institute of Agriculture of the Central-Chernozem zone, Voronezh, Russia 2Stony Steppe experimental forestry, Voronezh, Russia 3All-Russian Research Institute of Forest Genetics, Breeding and Biotechnology, Voronezh, Russia Abstract: Studies conducted in Dokuchaevsk agroforestry complex. The soil cover is characterized by a significant diversity and the nature of moisture. In this regard, the aim of the research was to assess the formation of forest stand depending on soil moisture. The materials of the carried out taxations of forest strips during the last century were used. For all test sites, a detailed analysis of the species composition, number of trees and growth parameters of the middle tree of the first tier is given. The average volume of wood supply stem wood per 1 ha was calculated on a single table "Forest of the subsidiary books." The processes of growth and development of tree crops in old-age plantations are shown. The features of the forest biological processes occurring in the protective forest plantations created by the foresters of the special expedition of prof. The influence of the regime of groundwater level on the growth parameters of the tree stand components. The dependence of growth and development of tree species on the distance from the main underground flow has been revealed on the basis of the materials of the forest strip inventory. On the formation of stands in different conditions, soil moisture has more influence than the proportion of oak in planting schemes of mixing. Analysis of the composition of the first tier stands shows that over the past 70 years on the Central segment of the formula composition of the stand 6D, 2Яо, 1Яп, 1V+Ko. Forest inventory in 1936 (age 41) it was 6D,2Яп,1Яо,1V. Formula of woody composition has changed slightly.  

To the future without poliomyelitis: the state of the epidemic, vaccination issues (review) Belova Olga Evgenevna FBRI SRC VB «Vector», Koltsovo, Novosibirsk reg., Russia   Abstract:  Polio is a highly infectious disease caused by a virus. It invades the nervous system, and can cause total paralysis in a matter of hours. Among those paralysed, 5% to 10% die when their breathing muscles become immobilized.The review analyzes the epidemiological situation of poliomyelitis in the world, describes the progress and risks associated with vaccine-derived polioviruses, the existing immunization tactics and the prospects for creating new safe and effective vaccines - genetically stable oral polio vaccine and available inactivated polio vaccine, innovative vaccine delivery and administration technologies, Studies conducted in this direction. The importance of detecting and terminating transmission of poliovirus, improving epidemiological surveillance and response to outbreaks, planning for efforts to ensure a poliomyelitis-free world after poliovirus elimination, including poliovirus containers, population protection, detection and response to sudden, recurrent outbreaks of poliomyelitis, is underlined.  

Comparison of Supervised Classification Techniques and Gaussian Mixture Distribution Method for Estimating Land Use Type on the Landsat 7 Satellite Data The applications of remote sensing on the land cover classification are very important for lands planning. . For this purpose multispectral satellite data uses in agricultural areas due to practically in large-scale. Many of the studies have been carried out with supervised classification based on pixel function. However, it is not possible to determine what kind of uses are in the land where the natural pattern distribution is unknown. For this reason, some statistical approaches and conditional algorithms are trying in the classification. The most common of these is Gaussian mixture distribution analysis (GMD). Mixture distribution analysis is multivariate statistical methods that deal with the separation of objects or different clusters of observations and the re-organization of pre-defined groups. This study was conducted for classification of sugar beet and wheat with grassland by using SC and GMD on the Landsat 7 ETM+ data in 1850 ha areas of Konya region and the spatial ranges were compared with the location realities. The result of calculations has shown the SC estimated sugar beet, wheat and grassland with accuracy coefficients at 0.88, 0.90, 0.83, respectively. On the other hand, GMD predicted with accuracy coefficients at 0.94 for sugar beet, 0.96 for wheat and 0.92 for grassland. As a result, GMD can be used with high accuracy in the detection of different land cover types in unknown lands. However, similar studies should be performed on different types of land cover for creation of spectral libraries, thus GMD could determine all of the land cover types.

Influence of herbicides on the productivity of maize in the conditions of ordinary chernozems in the Rostov Region Eng. Influence of herbicides on the productivity of maize in the conditions of ordinary chernozems in the Rostov Region  Grinko A.V., Polienko E.A. Federal Rostov Agricultural Research Center, Rassvet, grinko82@mail.ru  Abstract. One of the most important ways to increase the yield of corn is to control weed vegetation using chemical protection preparations. It is necessary to assess the level of harmfulness and selection of herbicide. The preparation should correspond to the floral composition of the weeds. In the Rostov region in many cases, the use of herbicidal preparations in the form of tank mixtures is optimal, therefore 6 variants of herbicides from different chemical classes were chosen as the objects of the study. As a result of the research, it was found that the use of combined herbicidal preparations containing several active substances is most effective. This allows to significantly expand the range of action of herbicides and increase their efficiency and payback.  

The specific of degraded pastures' formation while climate change in the Western Pricaspiy Gasanova Zarema Ulubiyevna, Zagidova Raisat Murtuzaliyevna Precaspian Institute of Biological Resources of Daghestan Scientific Center RAS, Russia, zgasanova@list.ru  Steppening of pastures in the Western Caspian has smoothed a little the natural factors of soil and vegetative cover degradation, but the problem of degradation remains relevant due to anthropogenic pressure increasing by small horned livestock overgrazing and raising the level of saline groundwater. The purpose of the study is to assess the influence of climate on the example of light chestnut and meadow saline soils and vegetation. Within the Terek-Sulak lowland the horizon A has been reduced in light chestnut and meadow solonchak soils for 50 years, the salt content in soils has increased two-three times. Long-term impact on pastoral ecosystems of natural and anthropogenic factors have led to significant changes in the indigenous meadow and meadow-steppe communities. In plant associations there is a decrease in the quantity of valuable species of fodder forage (Agropyrum). Restoration of pastures is mainly due to feather grasses having a large mass and distributional areas. In the Terek-Kuma lowland an increase in the amount of precipitation for 20 years has stimulated the optimization of light chestnut soils located on the dominant elements of the relief, in the upper 28-cm thick the salt content decreased. On degraded areas of light- and medium loamy light-chestnut soils, there is an increase of one-year-old saline groupings and ephemerals. In the cereal and wormwood associations increased the participation of feather grasses (Stipa), valuable in the fodder of Camphorosma. On eroded areas of sandy loamy chestnut soils "self-melioration" is observed: in the abandoned roads and blowing basins Salsola Kali ("roll-field") accumulates, where it breaks up, creating an anti-deflation cover. On meadow solonchak soils, the content of salts increased two to five times, the participation of one-year-old saline plant associations increased.

Soil-ecological state of technogenic landscapes of Novokuznetsk industrial complex Dvurechenskiy Vadim G., Androhanov Vladimir A. Institute of Soil Science and Agrochemistry, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; dvu-vadim@mail.ru Abstract: The article reflects the features of the formation of the soil cover of technogenic ecosystems that form on various substrates. Based on the results of soil mapping, an assessment of the soil-ecological state of technogenic landscapes of the Novokuznetsk industrial complex located in the south of Western Siberia in the Kemerovo region is given. Some variants of reclamation are offered. As a result of recultivation work and natural restoration of vegetation, young soils are formed on the surface of man-made ecosystems - embriozems and technozems. The quality of such soils and coverage area characterize the soil-ecological state of the ecosystem. The soil-ecological state of the Baydayevsky coal mine is considered good, since for the last 20 years the most evolutionarily developed embryozems humus-accumulative and technozems humusogenic have been diagnosed on its surface. The soil-ecological state of the ash dumps of the Tom-Usinsky State District Power Plant and the tailing dumps of the Abagursky concentration factory is considered unsatisfactory, since for a long time (about 20 years), the development of the soil cover has stopped at the initial and organo-accumulative stage, in connection with the properties of the substrate. The physicochemical characteristics of different types of soils in man-made landscapes revealed that the most qualitative changes in physicochemical properties occurred in embryozems and technozems formed on a coal-cut dump; less qualitative - on tailing dumps and ash dumps. Therefore, it can be stated that the man-made substratum composing ash dumps and tailings is less suitable for carrying out biological recultivation. Without the recultivation works of the land reclamation plan aimed at creating an artificial root layer, these facilities will have a negative impact on the ecological situation in the area of ??their location for a long time.

MicroRNA: markers of male infertility. The molecular basis of dysfunction and pathogenesis of male reproductive system are not fully known at the moment, but the results of several researches revealed that microRNAs play an important roles in precise regulation of post-transcriptional processes in spermatogenesis. It is involved in immune reactions, thermal and oxidative stress responses, it directs effects on proliferation and differentiation, chromatin compactification, apoptosis, affects the synthesis of functionally important proteins in the metabolism of spermatozoa, the synthesis of proteins involved in sperm motility, et cetera. All these facts are sufficiently confirm that differentially expressed in normal and pathological processes of sperm production  microRNAs can be used as reliable markers for different variants of patospermia. Considering that the spermatogenesis disturbance is one of the most significant cause  of male infertility, identification of specific microRNAs and studying of its regulatory mechanisms are very important for the formation of through understanding of the problem of male infertility and for the development of therapeutic methods. So, the main purpose of this article is compilation a literature review over the past six years on the topic of altered expression of microRNAs in seminal plasma, testicular tissue in several types of spermathogenesis abnormalities. In generally, analysis of the literature sources has shown that miR-15, miR-126, miR-449, miR-514, miR-517, miR-1260 are down-regulated and miR-99, miR-141, miR-193, miR-373, miR-429 are up-regulated in various pathologies of spermatogenesis. Expression profiling of the following microRNAs in various pathologies of spermatogenesis have also been repeatedly noted by researchers, but the concentration of each of them could be increased and decreased depending on the type of pathospermia: miR-16, miR-19, miR-26, miR-29, miR-30, miR-34, miR-122, miR-125, miR-181, miR-374, miR-509, miR-574.    

Model operation of efficiency of crop rotations in the conditions of the forest-steppe of Western Siberia Alternation of crops, will remain a fundamental element of zonal technologies of their cultivation for a long time. Application of methods of mathematical model operation will allow to come to new level in the solution of the questions of effective use of an arable land, through application of models optimizing it. Results of researches which basis data of long-term observations are will be especially valuable. They used data of the long-lived stationary experiments on studying of alternation of crops in crop rotations of last more than five rotation. Stationary experiences are located in a forest-steppe zone of Western Siberia on the fields FGBNU "SIBNIISKH". The purpose of researches consisted in optimization of use of an arable land at application of methods of mathematical model operation for preservation of soil fertility. The model allowing when performing particular conditions is given in work (balance of a humus, work expense, a metabolic cost, etc.) to optimize use of an arable land against the background of preservation of soil fertility of the soil, receiving production, economic and power efficiency. It is shown that at alternation of grain crops with clear steam, formation of a harvest happens due to natural fertility of the soil what the negative balance of a humus which reaches 0,18 – 0,78 tons from hectare testifies to. At alternation of field cultures without clear steam the harvest was formed against the background of reproduction of organic matter of the soil, due to increase in vegetable oddments from use of fertilizers, the balance of a humus was positive – 0,15 – 0,28 tons from hectare. Use along with clear steam, busy steam, colza on green fertilizer, and also straw as organic fertilizer for grain crops, 2,01 tons from hectare of commodity grain were received, at a share of clear steam – 16,8%, busy steam – 11%, summer grain – 72,2% of arable land. Increasing in crop rotations of Zapadnoy Sibiri Square with use as organic fertilizer of straw, busy vapors and green udbreniye, it is possible to achieve reproduction of fertility of the soil with some decrease in an exit of commodity grain to 2 tons from hectare.    

Effect of Combined Magnetic Fields with Very Weak Alternating Component on Duration of Pupal Metamorphosis Stage in Mealworm Beetle Tenebrio molitor It is shown that an exposure of pupae of the mealworm beetle Tenebrio molitor to the combined static (42 µT) and very weak extremely low-frequency alternating collinear magnetic fields exerts different influence, depending on the frequency of the alternating magnetic field (AMF), on duration of metamorphosis processes in these insects. For instance, a no less than 8 hour-exposure of young pupae to weak combined magnetic fields, adjusted to the frequency of ion cyclotron resonance for Ca2+ (32.2 Hz) at the AMF of 100 and 250 nT, inhibits metamorphosis process – a transitional stage from pupae to imago lasts longer. In contrast, a stimulating effect was observed when adjusted to cyclotron frequency for ion of glutaminic acid (4.4 Hz) at the amplitudes of 40, 100 and 250 nT. At the weaker amplitudes of the AMF this effect is not seen. For instance, an exposure at a frequency of 4.4 Hz, the amplitude of AMF 6, 16 nT and at a frequency of 32.2 Hz, the amplitude of AMF 6, 16 and 40 nT exerts no noticeable effect on the duration of the pupal metamorphosis stage.

Poliomyelitis: on the way to eradication In the pre-vaccine era, poliovirus was the main cause of disability in children. Today, the endemic transmission of poliovirus occurs in some areas of only three countries - Pakistan, Afghanistan and Nigeria, the number of polio cases has declined by more than 99 %. Of the three strains of wild poliovirus, only Type 1 continues to be detected. But the risks associated with unprotected low-level transmissions remain, which threatens the return of poliomyelitis epidemics in these countries and the spread of the virus to other countries in which poliomyelitis has been eradicated.The aim of this work is to analyze the state of the epidemic of poliomyelitis in Russia and in the world, the progress made in interrupting transmission of poliovirus and preventing outbreaks of circulating poliovirus vaccine origin (cVDPV); a review of the existing poliomyelitis vaccines and researches in this area (including the development of vaccines that do not contain live virus), vaccination problems in the final phase of elimination.The article highlights the importance of monitoring the maintenance of immunization coverage rates for children in decreed ages, the detection of type 2 polioviruses (wild, vaccine-bearing or vaccine-derived (Sabin strain) after a global transition from trivalent oral polio vaccine to bivalent OPV, discontinuation of oral poliomyelitis vaccine to eliminate rare long-term risks of the appearance of cVDPV and other measures to prevent the occurrence of cases of vaccine-associated poliomyelitis; a growing role for the environmental surveillance system; the need to continue fundamental research on poliovirus as a model system of other similar viruses that pose a threat to health.    

The relationship the biochemical composition of artificial nutrient media, morphological and physiological indicators of larvae great wax moth (Galleria mellonella L.) The article presents the research to explore the relationship of biochemical composition of the studied artificial nutrient media and morphological, physiological indicators of larvae of Galleria mellonella. To explore this issue was taken up widely used artificial nutrient media. This artificial nutrient media includes such ingredients as flour, dry milk, brewer's yeast, honey, glycerin, bran, beeswax, water. Material studies are the greater wax moth larvae (Galleria mellonella L.) and artificial nutrient media, which prepared according to the recipes of different authors. Statistical analysis showed that the significant relationship of the studied biochemical and morphological indicators. Calculation of correlation of the analyzed indicators have revealed a strong positive correlation between the content of initial moisture in the larvae and their survival (+0,80). The maximum initial moisture in the larvae observed in the experimental group according to the recipe of V. Ya. Ismailov and others № 1 of 68,05 %, while the survival rate in this group was 84,17 % by 11,44% above the control. Also the average negative correlation was revealed between ash content and mass of larvae (-0,66). In addition, the average direct correlation was revealed of the content of crude protein in the feed and crude protein in larvae (+0,64). Noted that the initial moisture of larvae and crude fat in the artificial nutrient media has a reverse correlation – -0,71. Thus, the artificial nutrient medium, balanced in protein, fat and carbohydrates, significantly increases the biological capacity of the insect. Recipe of Russian scientists E. M. Shagov, I. G. Ulanova, E. M. Aslanyan  [7] is selected from considered artificial nutrient media on a number of morphological and physiological indicators.

The involvement of microsatellite AGC in population-genetic differentiation of cattle for resistance to infection by the virus of bovine leukemia Glazko V.I. 1,2, Kosovsky G. Yu. 2, Tagmazian A.A. 2, Glazko V.I. 1,2, 1Russian State Agrarian University – Moscow agricultural Academy (MTAA) named after K.A. Timiryazev, Russian Federation Timiryazevskaya Str., 49, Moscow 127550 2Federal State Budget Scientific Institution Center of Experimental Embryology and Reproductive Biotechnologies, 127422 Russian Federation, Moscow, Kostyakova str., 12, bld.4. Abstract:  The comparative analysis of the genetic structures of cattle groups that differ in bovine leucosis virus (BLV) infection, according to polymorphism of different lengths DNA fragments flanked by microsatellite inverted repeats (GAG)6C, (AG)9C, (AGC)6G (ISSR-PCR markers) was carried out. The animals were divided into three groups - free from infection (REID-BLV-) and two groups of infected ones: bearing antibodies to viral proteins and cell clones with insertions in genomic DNA of proviruses DNA BLV (RID+BLV+) and carriers of antibodies to viral proteins, but no cell clones with proviruses DNA BLV insertions (RID+BLV-). Identified involvement in intergroup differentiation of genomic DNA fragments of different lengths flanked by inverted repeat sequences of microsatellite (AGC)6G, which, according to the literature, is closely associated with retrotransposons. Genetic distances estimated according to such fragments, were almost two times more between groups of RID-BLV- and RID+BLV+, compared to the differences between the RID-BLV- and RID+BLV-. The involvement in population-genetic differentiation of cattle for resistance to BLV infection the mechanisms of intracellular protection from retrotransposition were discussed.   

Investigation of the activity some 8-aminoderivatives of 3-methyl- 7-?-hydroxi-?-isopropoxipropylxanthines at the experimental hyperlipidemia. Ostapenko А. А., Belay I. M., Romanenko N. I. We made research in analysis of the antioxidant features of the newly synthesized derivatives of 3-methylxanthine in experimental hyperlipemia in rats. The results of the computer prediction of the possible manifestations of the biological activities indicate that the majority of the analyzed compounds can influence on the different sides of lipid metabolism. Markers of the influence on the activity of lipid peroxidation and the complex antioxidant activity during administration  of derivatives 3-methylxanthine were studied. This screening has allowed to identify some patterns of connections between the structure and the direction and severity of changes in the antioxidant properties of the blood of experimental animals. In addition, prospect of further pharmacological studies of the effects of synthetic analogs of xanthine are confirmed by appropriate patents and informational letters.  

Methodical features of biotechnology reproduction of soil fertility to realize the po-tential productivity crop. This article describes the resource-saving technology  of crop cultivation based on the transformation of the soil structure and the plant root system, secures up to 25% of harvest, even at extremely dry seasons.

Influence of an electromagnetic field on phytochemical and anatomic properties of herbs The anatomic and phytochemical analysis of the medicinal vegetable raw materials collected near the high-voltage power lines is carried out. The samples collected in the reserved territory were studied as a control. A number of the features resulting from electromagnetic field influence on soils and vegetable organisms have been revealed. It was proved that the majority of biochemical reactions within vegetable organisms had been suppressed under the influence of an electromagnetic field. It was endorsed by both anatomic anatomic (considerable decrease in leaves of the studied objects of such microdiagnostic signs as quantity the Druze of oxalate of calcium, quantity the golovchatykh of hairs), and quantitative (decrease in the maintenance of the main groups of connections of the studied raw materials – flavonoids and polysaccharides) methods.

Pigment complex of needles Far-Eastern and local species p. Juniperus The author presents the results of research of content of photosynthetic pigments (chlorophyll a, b and carotenoids) in needles of introducent and local species Juniperus, growing in arboretum Mountain-Taiga station FEB RAS.  It is established that the amount of photosynthetic pigments of introduce species is inferior to the local species. These data extend the idea of the pigment complex of introduced coniferous species and can be used to diagnose the state of photosynthetic apparatus.

Rare-distributed ornamental-leaved shrubs in collection of Ufa Botanical Garden Results of many years introduction of 6 genus of rare-distributed ornamental-leaved shrubs in Ufa Botanical Garden are presented. The detailed morphological characteristics of each taxa are given end data on seasonal cycle of development are also presented. Recommendations on propagation of the species end cultivars as well as agrotechnics of their planting under conditions of Bashkir Cis Urals are proposed.

The results of the breeding work with decorative herbaceous cultures in Bashkortostan as an example the genus Paeonia L The article summarizes the results of many years breeding work with paeonia hybrida hort. in the Botanical garden of Ufa. The paper describes the main stages of work in this area, describing the new cultivars of paeonia hybrida hort.

Poliomyelitis: the state of the epidemic and prospects Key facts. Overview of the global epidemic and the epidemic in Russia, retaliatory measures. The tools available to date for polio eradication. Polio vaccination: past, present and future.

On the Abrau Peninsula in 2010 was established the State natural reserve "Utrish". That purpose is to preserve the unique natural complexes of dry subtropics  of the Black Sea coast of Caucasus – hemixerophilic relict ecosystems  in the Northern Mediterranean.  For its territory (Abramsom cluster), in the project documents was given 859 vascular plant species. As a Supplement to the flora of the reserve "Utrish" strictly within the boundaries of the reserve, for 2012 – 2015 years of research we have identified the whereabouts of the 179 species of vascular plants. Also from the preliminary list has been removed 124 species, which is not confirmed by herbarium collections. The result of the audit of all submissions received become the floristic reserve list, which includes 914 species of vascular plants. The peninsula Abrau in 2010 created the State Natural Reserve "Utrish" to preserve the unique natural complexes of dry subtropical Black Sea coast - gemikserofilnyh relict ecosystems of the Northern Mediterranean. For its territory (Abrausky cluster), in the project documents were given 859 species of vascular plants. In addition to the flora of the reserve "Utrish" strictly within the boundaries of the reserve for the 2012 - 2015 years of research we have identified the location of 179 species of vascular plants. Also from the preliminary list of 124 species has been removed, which is not confirmed by herbarium charges. The result of the audit of all submissions received was the floristic reserve list, which includes 914 species of vascular plants. The full list is provided in the PDF-file of this article.

30 years' Changes of Probabilistic and Information Characteristics of the Humus Content in Southern Chernozems in Western Siberia The analysis of transformation of the probabilistic-statistical distributions (PSD) of the humus content in southern chernozems at the studied territory in the whole, situated on the Priirtyshsky uval for the 30-year period revealed existence of the tendency to decrease in the humus content in the top layer and its increase in underlying layers of soil. Transformation of PSD of the humus content is followed by very essential reduction of statistical entropy up to 60% of initial value that speaks about essential change of a condition of chernozems in spite of the fact that taxonomically soils don't change. The analysis of information divergence showed that the revealed tendencies in a sandy loam version are expressed more strongly, than in the loamy soils. And the greatest transformation of PSD of the humus content for the studied period happened in a layer 30-50 cm due to increasing of the low boundary of the interval of variation. The revealed transformations of PSD are interpreted from the point of view of interaction of soilforming, climatic and landscape and exogenous processes.

Consequences of eco-toxicological effects of hazardous heavy metals compounds on the benthic communities of river ecosystems The paper presents the results of the analysis of long-term regime hydrochemical information about the hazardous heavy metals (mercury, cadmium and lead) content of the river waters and hydrobiological information about the structural organization of the benthic communities. The possible  changes assessment of the ecological status of river ecosystems was conducted. It is shown, that the variability of the quantitative indicators of the benthic communities is response to the transformation of the component composition of the aquatic environment river’s.  The strengthening processes of environmental regression of  benthic communities takes place in river ecosystems with a high level of contamination of the toxic heavy metals.

Study of the sensitivity of hospital S. aureus strains to the action of disinfectants Has been studied the sensitivity of strains of S. aureus, isolated from hospitals environmental objects (air of manipulation rooms, swabs from the surfaces of medical equipment, uniforms of the medical staff) to modern disinfectants. It was established that the highest activity possessed chlorine compounds, and means based on peracetic acid.

The method of evaluation of potato varieties for resistance to viruses It is proposed another estimation of varieties and breeding hybrids of potato with regard to their clonal origin and propagation.

The functional state of the organism of calves during cold method of cultivation. Кostesha Nikolay Yakovlevich, Makarevitch Veronika Gennadievna For  the experiment 2 groups of calves were taken. An experimental group of  5 days old calves was put into individual cabins where the temperature was minus 4-5C. The control group was put into similar  cabins where the temperature was 11-12oC. The test of peripheral blood  in the experimental group showed that the quantity of erythrocytes increased significally compared with the control group of calves. The authors believe that moderate stress (according to the increase of cortisol in peripheral blood) stimulates erythropoiesis. Since the 7th  day of the experiment the calves began to gain weight up to 25% compared with the control group.

Evaluation of the rate constants reactions of the alkyl, allyl and aryl radicals with hydrocarbons with use the artificial of neural network Evaluation of the rate constants reactions of the alkyl, allyl and aryl radicals with hydrocarbons with use the artificial of neural network Tumanov Vladimir Evgen’rvich This paper discusses the use of feed-forward artificial neural network to predict the rate constants of organic molecules in the bimolecular radical reactions R + R1H in the liquid phase on the experimental data. The hybrid algorithm of calculation of rate constants of bimolecular radical reactions on the experimental thermochemical data and an empirical index of the reactionary center is offered. This algorithm uses an artificial neural network for a prediction of a classical barrier of bimolecular radical reaction at a temperature of 298 K, a database of experimental characteristics of reaction and Arrhenius's formula for calculation of a rate constant. Results of training and a prediction of the network are discussed. Results of comparison of logarithms of the calculated and experimental of the rate constants are given.

Clinical and epidemiological characteristics of rotavirus infection  infants This paper deals with epidemiological analysis of rotavirus gastroenteritis in infants in the first year of life and the study of nosocomial spread of diseases among them in the hospital. Shown widespread rotavirus gastroenteritis among children first month of life, the possibility of a wide nosocomial rotavirus infection children. The results obtained allowed for more effective prevention and control measures in the foci of infection.

Alexandr Fedorovich Lebedev —  first soil scientist Rostov University The article describes the main stages of the life and work of an outstanding representative of Russian genetic soil science Alexandr Fedorovich Lebedev. The authors attempted to collect anecdotal information about the first soil scientist Rostov University, who worked within its walls long before the organization of the Department of soil science.

Ecological State of Dark Chestnut Soil in Agrogene-transformed Ecosystems of Orenburg Zauralye The dark chestnut soil in various farmland are investigated in Orenburg Zauralye. The comparison of soil with virgin analogues showed that the degree of agrogenic transformation and environmental degradation of these soils increases in the following series: virgin — hayfield — grazing areas — fallow land — arable land.

Identification of fish species of the family of whitefish, harvested on the North Yenisei With the implementation of raw fish in the settlements of the Yenisei North revealed falsification of fish whitefish family, which is expressed in a specific regrading. During the study, the analysis of the scheme regrading Arctic cisco, Siberian whitefish and muksun produced in the lower reaches of the river basin Yenisei. Proposed a rapid method of identification based on knowledge of the morphometric characters.

Features reovirus RNA isolation from clinical material S. A. Kolpakov, E. P. Kolpakova. Key objective of the given research is to find out reasons of failures during extraction of the RNK reoviruse from sick people clinical material. In due course it was discovered that transcriptase (RNA dependent RNA polymerase) very quickly transforms double-stranded RNA into a single-stranded RNA due to short time of activation after violation of reoviruse outside capsid integrity. This single-stranded RNA is invisible using standard way of electrophoresis registration. Addition of a transcriptase with polymerase function in ready-made samples or in clinical material from sick people before RNA extraction leads to transformation of a single-stranded RNA (if available) into a dsRNA identified after electrophoresis in the form of genome’s segments. The developed RNA visualisation method could assist in etiological diagnostics of reoviral infection and in studying of reovirus as a pathogen.

New group of human rotaviruses, the family Reoviridae S. A. Kolpakov, E. P. Kolpakova. Key objective of the given research is to find out reasons of failures during extraction of the RNK reoviruse from sick people clinical material. In due course it was discovered that transcriptase (RNA dependent RNA polymerase) very quickly transforms double-stranded RNA into a single-stranded RNA due to short time of activation after violation of reoviruse outside capsid integrity. This single-stranded RNA is invisible using standard way of electrophoresis registration. Addition of a transcriptase with polymerase function in ready-made samples or in clinical material from sick people before RNA extraction leads to transformation of a single-stranded RNA (if available) into a dsRNA identified after electrophoresis in the form of genome’s segments. The developed RNA visualisation method could assist in etiological diagnostics of reoviral infection and in studying of reovirus as a pathogen.

Microbiocenosis of cavity uteri in missed abortion Studied complex microbiological and morphological study of endometrial scrapings 244 women with missed abortion. In the study found the prevalence of mixed viral and bacterial infections. Frequent combination of viruses marked with mycoplasma, ureaplasma and chlamydia.

Viral safety of blood products Grehirchak Nataliya Nykolayvna, Lych Inna Valentynovna The article deals with issue of ensuring viral safety of blood products. We defined responsibility and rightness of using method of polymerase chain reaction during production of blood specimen, appraised the quality of products for their promotion. The definition of contamination materials (plasma and precipitations), intermediate metabolite was carried out by method of PRC on the content of nucleic acid parvovirus B19, hepatitis B virus, hepatitis C virus in order to confirm the possibility of using abovementioned methods for quality assurance of blood components.

Problems and challenges of soil science application to developing information technologies in agricultural production of Rostov region Golozubov Oleg Modestovich, Kol’chik Anatolij Fyodorovich, Kryshhenko Vladimir Stefanovich, Litvinov Yurij Alekseevich The achievements, problems, and challenges of the modern stage of the development of the GIS for agromonitoring of agricultural lands in Rostov oblast. The structure of the information system of the GIS as an internet-based resource and its application for solving practical problems are described. For the first time in Russia, the GeoRSS standard based on the structured hypertext representation of the geographic and attribute information has been applied in the state system for the agromonitoring of agricultural lands in Rostov oblast and information exchange through the internet.

Resistance of microorganisms to antimicrobial drugs Pokudina Inna Olegovna, Shkurat Mihail Alekseevich, Battalov Dmitrij Valentinovich Abstract: The emergence of the phenomenon of resistance to therapeutic drugs in the pathogenic microorganisms, has a very negative effect on the health of humanity, because it leads to a sharp decrease in the efficiency of etiotropic therapy  of infectious diseases. Intense selective pressure of antibiotics leads to the rapid evolution and spread of new resistance mechanisms in health care settings and, above all, in the Intensive care unit. The article analyzes the literature data about the mechanisms of emergence and transmission of the resistance of pathogens to antimicrobial agents and acquired resistance associated with the production of beta-lactamases extended spectrum.

The search of probiotic culture of Bacillus subtilis on medicinal herbs’ biomass using the phages The culture of Bacillus subtilis witch can include probiotic strains was identified on the biomass of herbs (mint, camomile, milk thistle) using specific bacteriopfage. The authors suggest that medicinal herbs possess useful properties not only because of the biologically active substances, but also due to the content of enhancing therapeutic effect Bacillus subtilis in their decoction.

The study of microbial activity of multicomponent of eye drops for the treatment of conjunctivitis Microbiological activity of model mixtures of eye drops for the treatment of complex action conjunctivitis in cultures opportunistic pathogens St. epidermidis and Candida albicans oftalmomikozov been studied. The sterility of test solutions of eye drops has been studied. The zone destruction of microorganisms has been established.

Determination of the spectrum of lytic activity of bacteriophages by the electro-optical analysis of cell suspensions Changes in the electrooptical (EO) properties of cell suspensions of the Azospirillum brasilense strains Sp7, Cd, Sp107, Br14, KR77, S27, SR55, Sr75, Sp245, Jm6B2, S17, A. lipoferum strains Sp59b, Sr65, RG20am A. amazonense Am14, A. halopraeferans Au4, A. irakense strains KBC1 and KA3 d) in their contamination with the bacteriophage ΦAb-SR75 were studied depending on the infection. Control experiments were carried out to infect the cells with the bacteriophage, by making a standard inoculation of the dense nutrient media. The fact that the method for EO analysis of cell suspensions may be used to determine the spectrum of lytic activity of bacteriophages is first shown.

Strategy and tactics for control of viral diseases of plants for example potatoes The article offers a conceptual approach to the problem of control of viral diseases of plants. It is a selection of key strategic measures, taking into account biology of the host plant and features of viral infection. These include preventive measures related to seed production in virus-free basis, breeding for virus resistance and biotechnological methods of health promotion and propagation of virus-free material. These areas are mainly associated tactics of its receipt.

Broncho-Pulmonary Pathology as Leading Cause of Hospitalization and Death of HIV-Patients in Providing Urgent Medical Care at Multifield Medical Prophylactic Institution The causes of hospitalization and mortality of  HIV-infected patients (n=20) during 10-year period of medical treatment in a multidisciplinary health care setting (Military Medical Academy) were analyzed. Today the main causes of these persons death in these institutions are the diseases not associated with HIV infection: burns, combined injuries, toxic substances poisoning. It was found that HIV infection worsens the prognosis of patients hospitalized with respiratory apparatus diseases, promoting the earlier development of nosocomial infections, exacerbation of  broncho-pulmonary diseases and short-term mortality.  

Phytosanitary situation in a potato crop associated with the spread of viral diseases in the Astrakhan region The article describes the phytosanitary monitoring in a potato crop in the Astrakhan region. The materials obtained have shown that the strict implementation of previously recommended preventive measures to protect the normalized 2011 phytosanitary condition of potato fields with plantings associated with virus and phytoplasma infection in the Astrakhan region. However, in 2012—2014 years again, a difficult situation in terms epiphytotic viral infection.

Assessing the current state of the epidemic spread of HIV infection in the city of Magnitogorsk, Chelyabinsk region The purpose of this study is to evaluate the epidemic situation in the incidence of HIV infection in the city of Magnitogorsk, Chelyabinsk region, to identify the main features and trends in the epidemic. The epidemiology of HIV infection in Magnitogorsk is a high intensity spread of the disease among the population with signs of generalization. Identified the following unfavorable trends in the spread of HIV infection: active involvement in the epidemic of female and age of the population; increase the spread of HIV among drug users and patients with infections, sexually transmitted infections; increase in the number of patients with clinically advanced stages of HIV infection; increase the hidden sources of HIV infection; high mortality rate among patients with HIV infection associated with tuberculosis.

Innovation vaccines against dangerous viral diseases The experience of the development of candidate mucosal plant-made vaccines of oral administration. As an example the data about the development of vaccines against HIV-1/AIDS, hepatitis B and cervical cancer are considered in the paper. Resuls of the induction of immune responses in mice after the vaccination with dried fruits of transgenic tomato plants containing antigenic proteins TBI-HBS, PreS2-S and HPV16 L1 are reported.  

Effects of viral infection on the protein composition of the thylakoid membrane of tomato, pepper and cucumber Cucumber mosaic virus (CMV) and Tomato mosaic virus (TMV) causing viral diseases in many important plants worldwide. Thylakoid membranes were isolated from chloroplasts of infected plants (cucumber, pepper and tomato) and analyzed the protein composition by electrophoresis on PAGE gradient gel (10–25%). The gradient electrophoresis of thylakoid membrane proteins of virus-infected plants was analyzed pepper and tomato plants infected with TMV virus and cucumber plant infected with CMV virus revealed that the content of polypeptide in the 47, 33, 29–24 and 17–15 kDa comprising the submolecular complex of photosystem II complex and oxygen evolving complex significantly reduced compared with uninfected plants that may be associated with structural abnormalities in the complex PS II.

Application of cellular technologies for treatment of viral diseases The review is devoted to a hot topic — application of cellular technologies for treatment of viral diseases, in particular for treatment of AIDS.

Virology Today: Integration of D. I. Ivanovslii’s Ideas into the Methodology of Science In his research on the tobacco mosaic virus D. I. Ivanovskii has introduced a new type of causative agent of an infectious desease and a new form of life. In this way he has layed the basis of a ne field of science, virology. Its structure of knowledge and the logics of development caused formation of new scientific areas that go beyond the scope of natural science. The cognitive model of virology as science has become the prototype of computer virology and has influenced the development of communication theory.

Food product as a source of viral infections The main routes of transmission of viruses that can enter the body through infected food were considered. It has been shown that viruses are resistant to adverse environmental conditions and may be stored for several months in food and environment. Common sources, which can accumulate enteroviruses, Coxsackie B5 virus, hog cholera virus (HCV), African Swine Fever virus (ASFV), FMD virus, poliovirus, rotavirus and echovirus are described.

The influence of vertically transmitted virus infections on the spinal cord injury progression in newborn children The work represents the results of the radiological, clinical and laboratory examinations of 380 newborn children with a personal history of the spinal cord injury. The optimal examination of the newly-born children with birth traumas has been worked out. The pathology of the birth spinal cord injury in children with clinical evidence of vertically transmitted virus infection has been analysed.

Immunopathogenesis and therapy of liver disease of viral etiology Motivated immune pathogenesis of HCV viral infection. The analysis of immune dysfunction in chronic viral hepatitis, and its deepening in the context of the standard therapy, also the possibility of correction of drugs cytokines. The results and prospects of the use of Roncoleukin when optimizing the treatment of patients with chronic viral hepatitis presented.

The epidemiological situation of West Nile fever in Volgograd region West Nile virus for the Volgograd region is the most important arbovirus infection. The observed decrease in the incidence of WNV from 2001 to 2006, in 2008 and 2009 years, reducing the number of severe disease is only indicative of a temporary regression of the epidemic process in the Volgograd region. In the Volgograd region there are prerequisites and precursors complications epidemiology of West Nile fever. The purpose held in the Volgograd region surveillance in West Nile is to provide the information necessary and sufficient for the analysis of the epidemiological situation, as well as to determine the direction of policy and action in public health management decisions.

Molecular detection of baculoviruses of beet webworm from the collection of All-Russian Institute for Plant Protection Two isolates of baculoviruses from beet webworm Loxostege sticticalis (Lepidoptera: Pyraloidea) deposited in the collection of entomopathogenic organisms as a granulovirus (GVLS-85) and a nucleopolyhedrovirus (NPVLS-90) have been subjected to genotyping using degenerate primers L8F2:L8R2 flanking a conservative region of late elongation factor gene lef8. Both isolates, tested independently to avoid sample cross-contamination, were found to belong to a single molecular haplotype. This haplotype is unique, showing maximum sequence similarity of 73% to Pieris rapae granulovirus (Genbank accession number JX968491) and other granuloviruses. No significant similarity to available sequences of nucleopolyhedroviruses have been found.

Detection, identification and quantitation of bocavirus among children with acute respiratory viral infections and gastroenteritises in Moscow Human bocavirus (HBoV)) causes acute respiratory virus infections and gastroenteritises in humans throughout the world. HBoV DNA detection and identification among children with respiratory infections in Moscow during 2005–2010 by means of reverse transcription with subsequent real time PCR with fluorescent probes showed the long-term average frequency 14, 5 ± 0, 7% for 2, 395 nasopharyngeal lavages (variations in a rang from 13, 5% до 17, 0%). One should note seasonal activity of the HBoV circulation during a year with 2 peaks in autumn-winter and early spring. Among infants and toddlers less than 1 year old the HBoV was associated with other respiratory viruses, whereas for children 2–7 years old the bocaviral monoinfection was prevailed. Gastroenteritises were diagnozed in 34, 3 ± 3, 7% clinical cases. The HBoV viral loads in 40, 5% samples were more than 105 genome-equivalents per 1 ml for the first days postinfection, whereas for mixed respiratory infections and for children with the HBoV monoinfection were less 103 genome-equivalents per 1 ml.

Studying cyto-anatomical changes in tissues of virus infected Solanum tuberosum and Nicotiana tabacum plants Virus impacts on affected by them potato plants appear in stunting, the plant cells deformation and changing their structure. In virus-affected potato tubers, reduces quantity and size of starch grains. Obtained virus-containing callus from the test-plants consist of elongated and malformed cells containing amorphous inclusions.

Analysis of the tick-borne encephalitis virus coding sequences We have conducted an analysis of tick-born encephalitis virus (TBEV) coding sequences that are available via international data bank (GenBank). Upon comparison of polyprotein structures of 113 TBEV strains from various sites from Europe and Asia we have detected genotype-specific combinations of aminoacid substitutions. It was shown that strains Buzuuchuk from Kyrgyzstan and 178–79 from Russia (Eastern Siberia) have unique genomic structures.

Orthobuniaviruses as main factor for emergency diseases This analytical paper deals with the systematic and taxonomy of orthobuniaviruses, small developed dangerous and new viruses Akabane, Aino, Schmallenberg, Cache Valley diseases, Oropouche fever, Iquitos. The significance of reassortment mechanism of their origin and diversification are discussed.

Spreading of virus diseases of fruit-trees and small fruit cultures and modern techniques for their sanitation The spreading of different viruses on varieties and rootstocks of pome fruit trees, stone fruit plants, and small fruit cultures has been studied. It was established that efficiency of sanitation technique from viruses depended on culture, virus species and therapy method. The best results for obtaining virus free material were achieved by means of complex application of in vitro chemotherapy and magnetotherapy.

Preservation of infectious activity of industrial strains of rabies virus stored at different temperatures The dependence of specific infectious activity on the cell culture model of rabies virus industrial (fixed) strains L. Pasteur and CVS from the duration and storage temperature was investigated. Viral suspension was produced by cell culture BHK-21 / C13 and frozen at -20 and -80°C. Analysis of the specific activity was performed after 1 week, 1, 2, 3, 6 and 12 months of storage. It was experimentally discovered that the temperature -80°C is more effective for long-term storage for the studied strains.

Lithium salt of acrydon-acetic acid as a substance for biomedical research The article considers a new antiviral and imunomodulating drug with extremely high bactericidal, and particularly virucidal activity against RNA and DNA viruses, mycocidal and algicidal effect. Ways of the effective use of the drug in the national economic products are discussed.

The hybrid forecasting model on the basis of intellectual agents The hybrid model on the basis of intellectual agents is developed for the task of infectious dynamics prediction. Intellectual agents are created as recurrent neural networks and interact on the basis of the Markov chain. In comparison with traditional mathematical models the hybrid forecasting model of infectious dynamics has following advantages: take into account epidemic’s numerous parameters, possibility of model’s scaling and setup for different types of epidemics.

Тhe frequency of the human papillomavirus in the population of the Rostov-on-Don In this study the detection frequency of human papilloma virus types with high carcinogenic risk among residents of Rostov-on-Don was analyzed. The frequency of HPV-positive samples among women was 40%, among men — 21, 5%. Detection frequency of human papillomavirus was highest among young persons up to 35 years. More than 40% of HPV-positive persons had an average concentration of virus which may cause the cell dysplasia.

Virus inhibitory effect of the complex interferon inducer of type I in vivo in experimental herpes infection Ativiral effect of the molecular composition (MC) yeast RNA-tilorone and 2, 7-bis[2-(diethylamino)ethoxy]-fluoren-9-on dihydrochloride (tilorone) was demonstrated on the experimental model of herpes infection in vivo. The reduction of mortality by 80 % was observed in the group of herpes-infected animals treated with the MC, and the average life expectancy increased by 1, 5 times compared to the control. A reduction of the level of herpes simplex virus replication in the infected animal brain tissue was identified as the result of MC activity. The molecular composition of the yeast RNA and tilorone showed the most significant anti-herpetic effect comparing to the officinal drugs — Virolex and рoly(І)-poly(C).

The founder of the doctrine of viruses (To the 150th anniversary of the birthday of D. I. Ivanovsky) Describes the main stages of scientific and pedagogical activity of the D. I. Ivanovsky. Main results of his research, which determine the undisputed priority of the scientist as the founder of the doctrine of viruses.

Study of the stability of collection of potato varieties to virus diseases in the forest-steppe of Ukraine Studied 630 potato varieties for resistance to the major viral diseases in the forest-steppe of Ukraine. Established that the visual assessment of diseases complicated by nonspecific symptoms of some viral diseases. Using immunological and electron microscopic methods were identified pathogens of viral infections — Potato virus M and Potato virus Y. Highlighted accessions resistant to potato leaf roll, leafdrop streak and wrinkled mosaic.

On a plant Phryma asiatica (Hara) Probat. for the first time is revealed the isometric virus with the following characteristics: diameter virions — 30–32 nanometers; molecular weight of a capsid protein — 41,4 ± 0,8 kD; TIP — 80оС; LIV — more than 7 days; DEP — 10-4. In a refrigerator (is not higher 4оС) the infectivity of the phryma sap proceeded not less than 20 days. Against a virus the antiserum is obtained about which titer has made 1/12800. The antigenic affinity with the tobacco ring spot virus is shown. According to these data the pathogen falls belongs to Nepoviruses.

The accumulation of toxicants in the body of bees and honey in the conditions of ecologically crisis areas of the Republic of Tatarstan The article is devoted to the study of contamination of honey bees and honey toxicants in oil-producing regions of the Republic of Tatarstan. The results of the accumulation of a number of toxicants in the body of bees and honey.

Monitoring of mushrooms Agaricus bisporus (J. Lge) Imbach: diagnostics and prevention of diseases We conducted research and experiments and we determined that the mushrooms (Agaricus bisporus (J. Lge) Imbach) infected with bacteria, viruses, microscopic fungi. We recommend prevention activities to fight infections.

The comparison of standard metabolic rate and respiratory quotient in Eurasian blue tit (Cyanistes caeruleus, Aves) during winter and summer Standard metabolic rate in the Eurasian blue tit during winter significantly lower than during summer. Respiratory quotient in the Eurasian blue tit during winter higher than during summer, but not significantly. Standard metabolic rate and respiratory quotient had well pronounced diurnal rhythms with minimum in the nighttime (from 2 a. m. to 5 a. m.) and maximum in the daytime. The maximum difference between standard metabolic rates during daytime and nighttime was 45 % during winter and 15 % during summer.

The effect of foliar feeding of micronutrients on the formation yield of spring rape seeds One of the promising directions in improvement of technology of cultivation of spring oilseed rape is the application of microelements. The results of the studies have revealed increasing the yield of spring rape seeds by 19.7 % with foliar feeding is examined boric and sulfur fertilizers.

The study of the allelic state of microsatellite loci of sugar beet (Beta vulgaris L.) The results of studies of the genetic polymorphism in varieties and hybrids of sugar beet, of which several were polymorphic, are presented. Five types of alleles at marker 086 GZM were identified and analyzed using SSR-analysis. Established genetic similarity between two pairs of hybrids through the test locus, may indicate their common origin.

Bayesian analysis of 2?2 contingency tables relative proportions A new standard expression for distribution of epidemiological risk ratio estimates has been found out using two by two methods of study. Comparing homogeneous groups the distribution is operational within wide-ranging conditions studying both rare and frequent events. Support value of the distribution formula will increase primarily in cases with small amount of the cases or controls observed. Using the derived distribution excess mortality from lung cancer of the Mayak PA workers was associated with radiation exposure with high reliability of the observation.

The comparative study of the influence of pathogenic factors on the formation of congenital and acquired neurosensory hearing loss The aim is to carry out a comparative analysis of pathogenic factors influence on the formation of congenital and acquired NHL and on the formation of complex pathologies and peculiarities of psycho-physiological status of the child. Сhildren with NHL have a physical developmental lag in the process of individual ontogenesis, which is manifested in the lack of functional maturity of somatic and visceral systems of the body, the simultaneous presence of different of anomalies of growth and development. The comparative analysis of the influence of the environmental pathogenic factors on the formation of the congenital and the acquired NHL showed that the condition of intra- and inter-hemispheric integration and the nature of spectral power density of the EEG ranges in children with NHL can act as a functional criterion for the pathology development.

Problems and prospects of the main biogenic elements intramolecular isotope correlations using as a new resource of different aetiology pathologies diagnostics It is shown that chemical elements isotopes fractioning in chemical and biochemical reactions is based on two fundamental kernel properties — the mass and the spin; differences between them can affect on both chemical reactions speed and reactive systems energy state. In some cases deviations in mass-dependent linear isotopic effect for most of uneven isotopes from hydrogen to uranium are observed. These fractioning peculiarities can be used: in living organisms — for early disease detection of different aetiology pathology; in abiogenous natural systems — for new methods of heavy elements isotopes enrichment development.

Formation of the liver in amphibian larval period on the example of the green toad (Bufo Viridis Laurenti, 1768) Describes the morpho-physiological characteristics of the emerging liver in larvae of the green toad (Bufo viridis Laurenti, 1768). Identified that studied the body performs the function of blood during larval development period. In the liver are formed elements of the blood of all lines. Describes he specifics of the organization and morphological changes of the body from developing larvae. There are some pathological changes in the liver and formed elements of blood.

Kasatik mistrue (Iris notha Bieb.) in Azov district of Rostov Province Results of the two Iris notha Bieb. cenopopulations studies in Azov district of Rostov Province in a valley of Vodinskaja gorge (river Tchumburka bassin), first registered in 2014 are presented. There are determined: population area, quantity, density, age structure of cenopopulations, seeds productivity, limiting factors.

Communication quantitative chromosomal abnormalities of spermatozoa and preimplantation embryos Modern genetic diagnosis allows to establish chromosomal nondisjunction not only in postnatal and prenatal periods, but also in germ cells and early embryos. Thus, the most common nondisjunction in sperm are 18 and sex chromosomes. The most common aneuploidy in preimplantation embryos are chromosomes 21 and 18. Win abnormal embryos was 65,2 %, which indicates that the most effective elimination of nonviable embryos in early preimplantation stage.

Characteristics of regenerative processes in the reproductive system of cows in disadvantaged on viral leukemia farms Reproduction of the herd at the epizootic troubles is a challenge for many farms. Special therapeutic approach to gynecological pathology is required for detection of viral bovine leukemia.

Characteristic of microbial strains resistant to white phosphorus Abstract White phosphorus is one of the most dangerous environmental pollutants. In our works, cultures of microorganisms growing in media with a content of white phosphorus up to 1 % were obtained for the first time. These cultures are unique, and they are demonstrate first ever example of the inclusion of white phosphorus in the biospheric circulation of the phosphorus element. The morphological, partly biochemical and taxonomic descriptions of cultures of microorganisms that carry out the biodegradation of white phosphorus are performed. This work is important because a unique set of characters is a characteristic of the strain. The fact of the existence of a new strain of a microorganism is established only from the moment of its accurate description. s a result of the studies, the species affiliation of the studied microorganisms was established. It was confirmed by amplification of fragments of the 16S rRNA genes (in the case of streptomycetes prokaryotes) and 18S rRNA (in the case of fungi eukaryotes), carried out by the standard PCR method, using data from the international NCBI database. New strains of Aspergillus niger and Streptomyces sampsonii species have been identified. The research results show that the biodegradation of white phosphorus can be carried out by microorganisms from various taxonomic groups. Currently, the described strains are deposited in the All-Russian collection of microorganisms and may be subject to patenting.

Показатели изменения разнообразия экосистем в районе Кон Куонг, провинция Нгеан, Вьетнам УДК 502.31 Показатели изменения разнообразия экосистем в районе Кон Куонг, провинция Нгеан, Вьетнам Нгуен Ван Хонг1, Данг Хунг Куонг2,3, Лай Винь Кам1, Вуонг Хонг Ньат1, Чан Тхи Ньунг1, Нгуен Фуонг Тхао1, Нгуен Тхи Тху Хиен1, Ле Ба Бьен1, Колесников С.И.3 1Институт географии Вьетнамской академии наук и технологий, Ханой, Вьетнам; nguyenhong.ig@gmail.com 2Российско-вьетнамский тропический научно-исследовательский и технологический центр, Вьетнам 3Академия биологии и биотехнологии им Д.И. Ивановского Южного федерального университета, Россия  Аннотация: Усилия по сохранению естественной среды обитания и среды обитания человека посредством сохранения окружающей среды, направленные на изменение их показателей, всегда предпринимались на протяжении многих лет. Целью данного исследования является количественная оценка изменений показателей экосистемы в районе Кон Куонг, провинция Нгеан, Вьетнам, с 2000 по 2015 гг. В этой области исследований также есть национальный парк Пу Мат, где богато биоразнообразие среды обитания, видов, генов, а также экосистем. В этой статье семь индикаторов измеряются с помощью программного обеспечения ГИС и Fragstats, в том числе индекс разнообразия Шеннона (SHDI), индекс разнообразия Симпсона (SIDI), количество патчей (NP), плотность патчей (PD), процент подобных смежностей (PLADJ), индекс перемежения и сопоставления (IJI) и плотность края (ED). Значение для каждого индикатора будет присвоено для каждого наблюдения, чтобы отразить характер экосистемы. Они будут сравниваться друг с другом и учитываться изменения в экосистемах лесных, сельскохозяйственных, искусственных и других. Метод тематических наложений и переходных матриц также использовался для точной идентификации изменений экосистемы, произошедших за рассматриваемый период. В результате было получено подробное объяснение и картирование динамики экосистемы. Эта работа может быть воспроизведена и превратилась в методологическую фазу, чтобы показать индикацию экосистемы, потому что она добавляет эталонное значение для изучения различий в характеристиках экосистемы. Результаты показывают, что различие в окружающей среде в основном связано с уменьшением естественности и увеличением сложности сельскохозяйственных экосистем и роста городов.

Styding morphometrical indicators of palisad aparatus Quercus robur L. и Quercus rubra L. in an urbanized environment (on the example of Kaliningrad city). Styding morphometrical indicators of palisad aparatus Quercus robur L. и Quercus rubra L. in an urbanized environment (on the example of Kaliningrad city). Petrova Natalia G1, Mandrik Vladlena A1  1Immanuel Kant Baltic Federal University, Institute of Living Systems, Universitetskaya St., 2, Kaliningrad, 236040, Russian Federation. E-mail: petrova_sov@mail.ru Abstract:  In recent time had increased the extremely negative human influence on green spaces in the urban environment.  Impact of anthropogenic pollution leads to changes in the appearance of plants and morphometric parameters. The most sensitive and informative organ of plants is a leaf, it reflects the negative impact of the environment. The article considers the influence of the urban environment (areas with high traffic load) on the change in the morphological parameters of the assimilation apparatus in two types of plants of the genus Quercus L., they are often used in landscaping of Kaliningrad city. The purpose of the research is – studying some morphometric parameters of the assimilation apparatus in two types of plants as the genus Quercus: robur  L. and rubra L. in conditions of urbanized environment (on the example of Kaliningrad city). Quercus robur L. local species, Q. rubra L. Invasive plant, introduced to culture (East Prussia) in the late 19th century. In the research were used different methods such as: general scientific methods (observation, description, measurement, analysis, generalization) and special methods of bioecological research (anatomical and morphological, statistical). The vital state of plants has been studied, fluctuating asymmetry of leaves, morphometric parametres of the assimilation apparatus (the value of annual growth, the size of the leaf blade, the number of stomatous per unit area and parameters of the stomatous apparatus ). Some of morphometric parameters of the assimilation apparatus Q. robur L. и Q. rubra L. in the conditions of urban environment, indicate an increase in xeromorphism, others point to its weaking. Both the increase and decrease in xeromorphism indicators in these species should be considered as adaptive responses of plants to negative factors of environmental pollution.

Re-inventory of the flora of the Starikovsky site of the "Rostovsky" reserve The studies were carried out within the framework of a project to determine the value of the Starikovsky site of the State Natural Biosphere Reserve "Rostovsky" and a possible change in the composition of the vegetation cover, in connection with which a re-inventory of its flora was required. Under the conditions of a strict reserve regime and intensified drought, global changes in the floristic composition took place - some species disappeared and others appeared. Some of the views have been redefined. New species have also been identified, including 49 for the territory of the Starikovsky site. As a result of the research carried out, a re-inventory of the flora of the Starikovsky site was carried out and a new annotated list was compiled, including 312 species of higher vascular plants. The main attention was paid to the species of vascular plants listed in the Red Data Books of the Russian Federation and the Rostov Region, of which there are 14: 7 species of wild higher vascular plants included in the Red Data Book of the Russian Federation were registered: Stipa pulcherrima C. Koch, S. zalesskii Wilenskiy,  Tulipa schrenkii Regel, Iris pumila L., Calophaca wolgarica (L.fil.) Fisch. ex DC., Eriosynaphe longifolia (Fisch. ex Spreng.) DC. and Bellevalia sarmatica (Georgi) Woronow; 7 more species listed only in the Red Data Book of the Rostov Region: Stipa sareptana A.Beck., S. ucrainica P.Smirn., Psathyrostachys juncea (Fisch.) Nevski, Tulipa biflora Pall., Crambe tataria Sebeok, Astragalus physodes L., and also A. calycinus Bieb. – var. multiflorus Novopokr., which differs from the Caucasian species in its reddish calyx at the top, longer peduncles and multi-flowered inflorescences with smaller flowers. It should also be noted that an abundant growth of lichens was noted in the Starikovsky site, including Cladonia convoluta (Lam.) Cout., Neofuscelia pulla (Ach.) Essl. and Cetraria steppae (Savicz) Kärnefelt, listed in the Red Data Book of the region.

Biological characteristics of the carp Cyprinus carpio Linnaeus, 1758 in the South-Eastern part of the Taganrog Bay and the Don. Biological characteristics of the carp Cyprinus carpio Linnaeus, 1758 in the South-Eastern part of the Taganrog Bay and the Don. Guskov Gleb E.1, Guskova Olga S.2 1 Federal State Budgetary Institution of Science "Federal Research Centre The Southern Scientific Centre of the Russian Academy of The Sciences" (SSC RAS), Rostov-on-don, Russia; gleb_guskov@mail.ru 2 The Azov-Black Sea Branch of Russian Federal “Research Institute of Fisheries and Oceanography” (“AzNIIRKH”). Rostov-on-don, Russia.   Annotation: Over the past 50-70 years, significant changes in the hydrological and meteorological conditions have occurred in the Azov-Don basin. As a result of anthropogenic impact on the river flow of the river As well as due to climate change, the natural spawning grounds of a number of fish species, including valuable commercial ones, have significantly decreased. Intensive fishing in the past years has led to a significant reduction in the ichthyofauna of this basin. The object of our study is the wild carp Cyprinus carpio Linnaeus, 1758. This is one of the most valuable commercial fish species in the Don river basin, which requires control over the state and distribution of its population. Taxon was quite common for the f the Don basin. In the mid-90's, about 3-5 tons of wild carp were caught annually in the course of fishing in the Don and areas. However, over the past decade, the number of wild carp has declined sharply. This paper analyzes the General state of the studied population in the Azov-don basin. To assess the biological characteristics of wild carp, ichthyological control catches were carried out in the waters of the Taganrog Bay and its near-Delta part, in the Don. To interpret our results and to get a complete picture of the state of the carp population, we analyzed literature sources covering the subject under study, as well as survey data of Amateur fishermen in the study area. The source and reasons for the introduction of carp — a domesticated form of carp-into the river and Bay are described. Genetic contamination of wild carp in the lower river Don and Taganrog Bay is suggested.

Progress and Challenges in Poliomyelitis Eradication. Vaccine evolution (review) Progress and Challenges in Poliomyelitis Eradication. Vaccine evolution (review) Belova Olga Evgen’evna FSRI SRC VB «Vector», Koltsovo, Novosibirsk reg., Russia  Abstract: Poliomyelitis is an infectious viral disease caused by three serotypes of poliovirus. It affects the central nervous system and can cause paralysis. There are two effective vaccines that protect against polio - inactivated (IPV), given by injection, and live attenuated oral polio vaccine (OPV). The vaccination campaign, before it was halted by the coronavirus pandemic, almost succeeded in eradicating polio. Since 1988, the number of wild poliovirus cases has decreased by more than 99%. But in recent years, efforts to eradicate poliomyelitis have been hampered by outbreaks of vaccine-derived poliovirus, in which OPV recipients have isolated mutant variants of Sabin's stool strains that could become pathogenic vaccine-derived polioviruses and circulate through the population, causing disease in unvaccinated people. Polio will only be completely eradicated when all sources of the virus, including wild poliovirus (WPV) and vaccine-derived poliovirus (VDPV), are eliminated. There are now more VDPV cases of polio worldwide than WPV, and continued use of Sabin's OPV has resulted in additional outbreaks of VDPV. To eradicate poliovirus completely, a safer, more genetically stable, live attenuated vaccine is needed. The article summarizes the progress in global polio eradication and current challenges. The desired characteristics and new technologies for the production of vaccines, which can be used in conditions of complete elimination of the virus, are considered. The progress in the development of improved OPV strains based on innovative strategies is analyzed.

Features of the immune response in co-infections and in combination with invasions. Review Khozeeva E.V.1, Tretyakova A.V.1, Zimina Yu.A.11 Volgograd State University, Volgograd, Russia; zimina.yuliya@volsu.ruDOI:10.18522/2308-9709-2022-42-2AbstractIn the 21st century, bacterial, viral and helminthic diseases are still a serious health problem for a large number of people, not only in developing countries, but also in countries with a high level of medicine. Different types of infectious agents can be transmitted through the same routes, often leading to infection by two or more types of pathogens. Human infection with multiple pathogens is much more common than single infections. Given that different scenarios of the immune response develop in response to different pathogens, the behavior of the immune system in the presence of pathogens belonging to different taxa and causing different pathological processes is interesting. There are situations in which co-infection with opportunistic microorganisms can become much more dangerous for the body than the primary infection. Even with the proper functioning of the immune system, most often, the body cannot cope with several infections at the same time, which often leads to complications. The review provides a summary of modern research on this topic, published in foreign and domestic scientific journals, mainly over the past decade. The mechanisms of the immune response of humans and mammals to the most common combinations of infections, as well as helminth infestations, are considered. An attempt was made to classify coinfections depending on the scenario of their mutual effects on the body. The following main scenarios have been identified: mutual strengthening of infections, strengthening of one of the infections, weakening of the infection combined with helminthic invasion, and asymptomatic course of coinfections. The epidemiological situation in the world provides scientists with the opportunity to obtain a large amount of practical data. Therefore, the article pays special attention to the results of the latest research on the participation of the COVID-19 virus in coinfections.