03.01.06 Биотехнология (в том числе бионанотехнологии)

Effect of different phytohormones on Austrian sage multiplication (Salvia austriaca Jacq.) Effect of different phytohormones on Austrian sage multiplication (Salvia austriaca Jacq.) Vasilchenko Elena V., Sereda Mikhail M., Varduni Tatiana V. Southern Federal University, Rostov-on-Don, Russia  Abstract: The influence of phytohormones such as 6-benzylaminopurine, naphthylacetic acid and indole-3-acetic acid on the Austrian sage multiplication (Salvia austriaca Jacq.) in an in vitro culture is discussed in the paper. Austrian sage is the medicinal, essential oil, melliferous and ornamental steppe herb, included in the Red List of the Rostov region. The study was conducted in the laboratory of cellular and genomic technologies of plants of the Botanical Garden. Seeds from the collection of the Botanical Garden of the Southern Federal University were used as the primary explant. For cultivation the Murashige and Skoog medium was used. Murashige and Skoog medium without the addition of phytohormones was used as a control. Evaluation of the multiplication efficiency was carried out after 21 days. The multiplication factor was calculated as the number of new shoots. The highest multiplication factor was observed in the medium with the addition of 0.5 mg / l of 6-benzylaminopurine and 0.1 mg / l of naphthylacetic acid. This concentration of phytohormones is recommended for micropropagation of Austrian sage. The concentration of auxins (IAA and NAA) 0.3 mg/l leads to a decrease in the efficiency of Austrian sage multiplication. The height of shoot was approximately the same and no statistically significant differences were found on all tested mediums. It should be pointed out that with a greater concentration of phytohormones physiological abnormalities was observed.

Innovation vaccines against dangerous viral diseases The experience of the development of candidate mucosal plant-made vaccines of oral administration. As an example the data about the development of vaccines against HIV-1/AIDS, hepatitis B and cervical cancer are considered in the paper. Resuls of the induction of immune responses in mice after the vaccination with dried fruits of transgenic tomato plants containing antigenic proteins TBI-HBS, PreS2-S and HPV16 L1 are reported.  

Morphogenesis and regeneration of inflorescence axis in culture Iris ensata Thunb. The results of anatomical-morphological regeneration-investigation in explants of inflorescence axis of some cultivars I. ensata Thunb. are presented there. Morphogenesis peculiarities and regenerative activity of the given type of explants are found out. It’s noted, that morphogenesis is carried out similar to gemmagenesis, passing by the stage of callus formation. Shoots, formed de novo, were of endogenous origin.

Characterization of cell growth and accumulation of phenolic compounds in suspension culture of Trigonella foenum-graecum L. The paper presents a characteristic of cell growth activity and synthesis of phenolic compounds in suspension culture of Trigonella foenum-graecum L. during its cultivation. It was shown that the suspension culture is characterized by the typical S-shaped type growth and high viability. Predominant types of fractions are single cells and small aggregates. The suspension culture accumulates phenolic compounds, mainly phenolic acids, throughout the growth cycle.

Orchids Rare of Tempertate Zone and Modern Methods of Their Effective Preservation in Artificial Conditions Sheyko E. A. Along with the traditional techniques of plant preservation ex situ biotechnological methods used for these purposes are becoming more and more important. These work investigations dealt with the development and improvement of the methods of isolated rare and disappearing orchid tissue culture in order to apply them in the system of plant resources preservation and procreation. As a result there were obtained callus cultures of generative organs of Himantoglossum caprinum (Bieb.) C. Koch и Ophrys oestrifera M. Bieb. For the first time there was found the interrelation between the callusоgenesis from explants of orchid vegetative and generative organs and ratio of the phytohormonal complex components at the specific stages of the ontogenesis that must be taken into account during the development of methods of these species microclonal reproduction.

Effect of different phytohormones on Austrian sage multiplication (Salvia austriaca Jacq.) Effect of different phytohormones on Austrian sage multiplication (Salvia austriaca Jacq.) Vasilchenko Elena V., Sereda Mikhail M., Varduni Tatiana V. Southern Federal University, Rostov-on-Don, Russia  Abstract: The influence of phytohormones such as 6-benzylaminopurine, naphthylacetic acid and indole-3-acetic acid on the Austrian sage multiplication (Salvia austriaca Jacq.) in an in vitro culture is discussed in the paper. Austrian sage is the medicinal, essential oil, melliferous and ornamental steppe herb, included in the Red List of the Rostov region. The study was conducted in the laboratory of cellular and genomic technologies of plants of the Botanical Garden. Seeds from the collection of the Botanical Garden of the Southern Federal University were used as the primary explant. For cultivation the Murashige and Skoog medium was used. Murashige and Skoog medium without the addition of phytohormones was used as a control. Evaluation of the multiplication efficiency was carried out after 21 days. The multiplication factor was calculated as the number of new shoots. The highest multiplication factor was observed in the medium with the addition of 0.5 mg / l of 6-benzylaminopurine and 0.1 mg / l of naphthylacetic acid. This concentration of phytohormones is recommended for micropropagation of Austrian sage. The concentration of auxins (IAA and NAA) 0.3 mg/l leads to a decrease in the efficiency of Austrian sage multiplication. The height of shoot was approximately the same and no statistically significant differences were found on all tested mediums. It should be pointed out that with a greater concentration of phytohormones physiological abnormalities was observed.

Innovation vaccines against dangerous viral diseases The experience of the development of candidate mucosal plant-made vaccines of oral administration. As an example the data about the development of vaccines against HIV-1/AIDS, hepatitis B and cervical cancer are considered in the paper. Resuls of the induction of immune responses in mice after the vaccination with dried fruits of transgenic tomato plants containing antigenic proteins TBI-HBS, PreS2-S and HPV16 L1 are reported.  

Morphogenesis and regeneration of inflorescence axis in culture Iris ensata Thunb. The results of anatomical-morphological regeneration-investigation in explants of inflorescence axis of some cultivars I. ensata Thunb. are presented there. Morphogenesis peculiarities and regenerative activity of the given type of explants are found out. It’s noted, that morphogenesis is carried out similar to gemmagenesis, passing by the stage of callus formation. Shoots, formed de novo, were of endogenous origin.

Characterization of cell growth and accumulation of phenolic compounds in suspension culture of Trigonella foenum-graecum L. The paper presents a characteristic of cell growth activity and synthesis of phenolic compounds in suspension culture of Trigonella foenum-graecum L. during its cultivation. It was shown that the suspension culture is characterized by the typical S-shaped type growth and high viability. Predominant types of fractions are single cells and small aggregates. The suspension culture accumulates phenolic compounds, mainly phenolic acids, throughout the growth cycle.

Orchids Rare of Tempertate Zone and Modern Methods of Their Effective Preservation in Artificial Conditions Sheyko E. A. Along with the traditional techniques of plant preservation ex situ biotechnological methods used for these purposes are becoming more and more important. These work investigations dealt with the development and improvement of the methods of isolated rare and disappearing orchid tissue culture in order to apply them in the system of plant resources preservation and procreation. As a result there were obtained callus cultures of generative organs of Himantoglossum caprinum (Bieb.) C. Koch и Ophrys oestrifera M. Bieb. For the first time there was found the interrelation between the callusоgenesis from explants of orchid vegetative and generative organs and ratio of the phytohormonal complex components at the specific stages of the ontogenesis that must be taken into account during the development of methods of these species microclonal reproduction.