03.01.06 Биотехнология (в том числе бионанотехнологии)

On the biological degradation of white phosphorus On the biological degradation of white phosphorus   1 Institute of Organic and Physical Chemistry named after A.E. Arbuzov. Kazan Scientific Center of the Russian Academy of Sciences. Arbuzov St., 8. Kazan, 420088. Republic of Tatarstan. Russia. E-mail: mindubaev-az@yandex.ru 2 Kazan (Volga Region) Federal University. University St., 18. Kazan, 420008. Republic of Tatarstan. Russia. Abstract For the first time different taxonomic groups of microorganisms (fungi, Streptomyces and bacteria) are inoculated on culture medium containing white phosphorus as the single source of phosphorus. On these media microorganisms grew and have not experienced phosphorus starvation. It is the world's first example of the inclusion of white phosphorus in the biosphere cycle of elemental phosphorus. The highest concentration corresponds to 5000 times excess of MPC of white phosphorus in wastewater. The comparison of the sequences of ribosomal genes of the fungus, steadily metabolizing the white phosphorus, with sequences of the GenBank database, allowed us to identify this microorganism as a new strain of Aspergillus niger, to which we have assigned the number A. niger AM1. Inoculation of A. niger AM1 in medium containing just two sources of phosphorus (phosphate and white phosphorus) demonstrated that P4 does not exhibit toxic properties in relation to this microorganism. In the presence of white phosphorus it grows at the same rate as in the absence thereof. This is the only example of the lack of white phosphorus toxicity to a living organism. Our previous studies have demonstrated the absence of white phosphorus toxicity for Aspergillus niger AM1. However, the toxic properties of the substances are of different nature. It is of great interest to study the genotoxicity – a possible source of mutations. In the present work SOS-lux test has demonstrated genotoxicity of white phosphorus. This result is obtained for the first time – all the available literature sources reported no genotoxic properties of white phosphorus. Allium test showed the mitotoxic effect of white phosphorus on eukaryotic cells. A very interesting and unexpected result was the discovery of a mutant Aspergillus niger AM1. In comparison with an ancestral strain this mutant possesses an unusual morphology and grows more intensively in culture medium with white phosphorus. Three strains of A. niger, sent from the All-Russian collection of microorganisms, also showed much higher resistance to white phosphorus than bacteria. According to preliminary data, resistance to white phosphorus the A. niger is fixed in the genome.

Resource-saving elements of soybean cultivation technology on the slopes of the Rostov region Resource-saving elements of soybean cultivation technology on the slopes of the Rostov region Rychkova M.I., Gaeva E.A., Mishchenko A.V. Federal Rostov Agricultural Research Center, Rassvet. Е-mail: emmaksay@inbox.ru. Abstract. The article presents the actual results of research on the development of resource-saving elements of soybean cultivation technology, providing moisture saving, high productivity and soil protection from erosion. Two-year studies were carried out on the erosion-dangerous slope of the South-Eastern exposure of the Bolshoy Log beam, Aksai district of the Rostov region in the system of contour-landscape organization of the territory in 2017-2018. Studied two methods of primary tillage – otvalnuyu adopted for monitoring and chisel. Chisel tillage in the cultivation of soybean was performed by chisel plow. Dump processing was carried out with a plow blade. The mineral nutrition system included three backgrounds: natural fertility recommended by zonal systems and increased by half. It is established that to prevent possible erosion it is recommended to cultivate soybeans in the system of contour-band placement of fields. It is shown that the payback of the crop fertilizers on the version with the chisel treatment of the soil is higher than on the dump. Increasing the dose of fertilizer in half reduces the payback of fertilizers by 18.9-19.4 %. It is revealed that the approved soil protection (chisel) tillage can significantly reduce the erosion processes – soil flushing by 22.9%, runoff – 10.0%, the cost of fuel and lubricants-18.8%, increase the accumulation of reserves of productive moisture in the soil with a more economical expenditure, yield – by 30.2 – 32.1%, conditional net income-up to 6.6 thousand rubles /ha, profitability-by 42.1%, recoupment of direct costs yield of 1.4 rubles per ruble in comparison with the same indicators at the dump plowing.

Location on the Caucasus Potentilla multi-advisable (Potentilla multifida L.) Location on the Caucasus Potentilla multi-advisable (Potentilla multifida L.)  O.N. Demina, L.L. Rogal'  Abstract: The paper describes the Gum-Bashi Pass, which is named after the homonymous right-bank tributary of the Kuban - the Mara River. The distribution of grass vegetation on the Gum-Bashi Pass, as on the whole Rocky Caucasus Mountains, is associated with vertical zonality or altitudinal belt ecosystems. The characteristic of indigenous subalpine vegetation is given: on gentle northern slopes and plateaus of the Rocky ridge formed lush grassy meadows; Petrophytic steppes develop on the steep slopes of the southern, southeastern, and southwestern exposures. The most common types of natural forage lands on the Gum-Bashi Pass are mountain meadow communities on mountain sod-podzolic, gray and brown forest soils, and meadow-steppe communities on leached and podzolized black soil. However, the main purpose of the work is to describe a new plant species, Potentilla multifida L., which we discovered for the first time in the territory of Karachay-Cherkessia, in the Rocky Ridge system on the Gum-Bashi Pass and confirming its growth in the Caucasus. In describing this species, it is mentioned that this herbaceous perennial reaches 40 cm in height. The leaves of the plant are green above and beloiclochic below, dissected into linear lobes. The flowers are small, on thin pedicels, pale yellow. The beginning of its flowering was recorded when the species was collected. A critical summary of references to this species and an indication of the need to describe a new Caucasian form is given. In conclusion, one more time focuses on the need for this description, studying the distribution of P. multifida in the Caucasus and its phytocenotic environment. The article is illustrated with a large number of photos.

Effect of different phytohormones on Austrian sage multiplication (Salvia austriaca Jacq.) Effect of different phytohormones on Austrian sage multiplication (Salvia austriaca Jacq.) Vasilchenko Elena V., Sereda Mikhail M., Varduni Tatiana V. Southern Federal University, Rostov-on-Don, Russia  Abstract: The influence of phytohormones such as 6-benzylaminopurine, naphthylacetic acid and indole-3-acetic acid on the Austrian sage multiplication (Salvia austriaca Jacq.) in an in vitro culture is discussed in the paper. Austrian sage is the medicinal, essential oil, melliferous and ornamental steppe herb, included in the Red List of the Rostov region. The study was conducted in the laboratory of cellular and genomic technologies of plants of the Botanical Garden. Seeds from the collection of the Botanical Garden of the Southern Federal University were used as the primary explant. For cultivation the Murashige and Skoog medium was used. Murashige and Skoog medium without the addition of phytohormones was used as a control. Evaluation of the multiplication efficiency was carried out after 21 days. The multiplication factor was calculated as the number of new shoots. The highest multiplication factor was observed in the medium with the addition of 0.5 mg / l of 6-benzylaminopurine and 0.1 mg / l of naphthylacetic acid. This concentration of phytohormones is recommended for micropropagation of Austrian sage. The concentration of auxins (IAA and NAA) 0.3 mg/l leads to a decrease in the efficiency of Austrian sage multiplication. The height of shoot was approximately the same and no statistically significant differences were found on all tested mediums. It should be pointed out that with a greater concentration of phytohormones physiological abnormalities was observed.

Methods of soil fertility preservation on eroded slopes of Rostov region Methods of soil fertility preservation on eroded slopes of Rostov region Gaevaia Е.А. Federal Rostov Agricultural Research Center, Rassvet. Е-mail: emmaksay@inbox.ru. Abstrac: Тhe Studies were carried out in a multifactorial stationary experiment in 1991-2018 years. the Experiment is located on the slope of the beam of the Big Log of Aksai district of Rostov region. The soil of the experimental site is ordinary heavy loamy Chernozem on loess-like loam, medium-eroded. In the experiment studied two crop rotation zernopererabotki (I) and sertralineprograms (II), and three levels of organic-mineral system of fertilizers («0» – natural fertility; «1» – cattle manure 5 t + N46P24K30 and «2» – cattle manure 8 t + N84P30K48 1 hectare). The aim of the study was to study the balance of humus depending on the design of crop rotations located on the eroded slopes of the Rostov region, to identify ways to preserve soil fertility at the initial level and increase it. As a result of the research, the amount of washed soil was determined. The importance of plant residues in the accumulation of humus is shown. It is established that the intensive use of agricultural land in the Rostov region without fertilizer leads to a reduction in the humus content in the soil. The role of perennial grasses in reducing soil flushing by 35.6% was studied. It is shown that the long-term use of organic fertilizers in crop rotations at a dose of 5 tons of cattle manure and N46P24K30, as well as the introduction of 20% of perennial in the crop rotation allows to maintain a positive balance of humus at the level of (+35 kg/ha) in the grain-grass crop rotation. Increasing the dose of fertilizer to 8 tons of cattle manure and N84P30K48 contributes to the accumulation of humus up to (+100 kg/ha) in the grain crop rotation. The possibility of replacing organic fertilizers with 1-2 t/ha of wheat straw using compensatory doses of nitrogen fertilizers is shown.

To the future without poliomyelitis: the state of the epidemic, vaccination issues (review) To the future without poliomyelitis: the state of the epidemic, vaccination issues (review) Belova Olga Evgen’evna  FSRI SRC VB «Vector», Koltsovo, Novosibirsk reg., Russia  Abstract: Polio is a highly contagious viral infection that affects mainly children under five years of age, which can lead to paralysis or even death. Damage to the nervous system is manifested by fever, pain in the limbs, then peripheral irreversible paralysis. In severe cases, paralysis of the respiratory muscles can occur, which causes death. The transmission mechanism is predominantly fecal-oral. The only way to combat polio remains vaccination. The vaccine provides the development of long-term humoral and local immunity. The purpose of this work is to analyze the epidemiological situation of poliomyelitis in the world 31 years after the adoption of the Global Polio Eradication Program, and to compare the characteristics of live and inactivated polio vaccines. Issues related to the use of live oral polio vaccine and the need for its phased withdrawal from circulation, the problem of vaccine-related polioviruses and vaccine-associated paralytic polio are considered. Describes ongoing research into the development of new safe and effective polio vaccines; approaches to the development of new IPV to reduce costs, increase coverage and reduce the risks of using live polioviruses in vaccine production; new technologies for the delivery and administration of IPV; clinical studies of new vaccines and vaccination schedules. The main objectives of the strategy to complete the eradication of poliomyelitis for 2019-2023 are discussed: eradication, integration, certification and containment.

The ameliorative condition of the soil spread around the Kattakurgan reservoir and its changes The ameliorative condition of the soil spread around the Kattakurgan reservoir and its changes Abdullaev S.A.1, Jabbarov Z.A.1, Tursunulova A.1, Okolelova A.A.2, Kholdorov Sh.M.3 1National University of Uzbekistan, Tashkent, Uzbekistan, zafarjonjabbarov@gmail.com 2Volgograd State Technical University, Volgograd, Russia, allaokol@mail.ru 3Tokyo University of Agriculture and Technology – TUAT, Tokyo, Japan, sh.m.xoldorov@gmail.com Abstract: Changes in the physicomechanical properties and ameliorative state of the soil were studied using the Kattakurgan reservoir as an example; the degree of fossil, the density of irrigated lands, the dynamics of the groundwater level by years, the ameliorative state and the location of salts by layers were studied. According to the results, the indicator of soils with an inadequate ameliorative state increased from 4.22% to 5.58%. It was justified that the level of groundwater salinity is mainly between 0-1 g / l and 1-3 g / l. It is scientifically substantiated that the main effect of the Kattakurgan reservoir on soils is formed by the accumulation of salts, the effect on the physicomechanical properties is relatively small.

170 years since the birth of V.V. Dokuchaev Bezuglova Olga Stepanovna Abstract: March 1, 2016 marks 170 years since the birth of  V.V. Dokuchaev – an outstanding scientist, naturalist, founder of soil science. His views, theoretical and practical research influenced the development of a number of related disciplines, as well as the later appearance of such sciences as the doctrine of the biosphere and biogeochemistry.   Dokuchaev's ideas to restore the fertility of chernozem  steppes, which were implemented, have slowered the rapid degradation of the soil, but the situation remains difficult. In this regard, one of the urgent tasks of modern soil science is to increase public awareness of the importance of soils for food security and the implementation of essential ecosystem functions.

Global warming and its regional implications for the European part of Russia The article examines trends in global and regional climate, the forecast of their likely future changes, and the possible implications of these changes for economic activity in that region.

Modification of the nutrient medium to produce a pure culture of mushrooms of genus Pleurotus We investigated creation of the nutrient medium for extracting a pure culture of Basidiomycetes of genus Pleurotus – perspective producers of physiologically active substances.

The role of shooting range on information content of ecosystems space pictures of lands of agricultural purpose Berezin Leonid Vladimirovich Abstract: The efficiency of the analysis of a range of the reflected solar radiation in space pictures of the grain crops and laylands, which are planned to repeated development, increases at a clustering on light reflection intensity. The spectral curve of the allocated classes is defined by a shooting range. Its maximum information content is reached at the stage of pictures synthesizing at a priority of infrared range of a range of reflection of solar radiation, in comparison with its visible short-wave part.

The influence of temperature factor on spontaneous contact interaction of lymphocytes with granulocytes Revealed the effect of temperature factor on spontaneous contact interaction of lymphocytes with granulocytes. It is established that this cellular interaction in vitro is implemented in the form of three different types, the intensity of which is related to the temperature parameters of the joint incubation of these cells.

Special features of highly technological processing of the tomatoes  In the food branches of the industry of many countries the tomatoes are considered as the most popular culture because of the valuable nourishing and dietetic qualities, the wide variety of types, high responsiveness to the methods of cultivation used. However, the state of technology and equipment for processing of tomato raw material existing at present requires further development and improvement.  Canning industry is the branch forming the budget of the republic of Daghestan. To 30  % the financial means of republic it comes from that processing, including from canning industry. However, according to the quality indicators and using the methods of packing the canning production, manufactured in the republic thus far cannot compete with the analogous production on the international market. In many respects the low competitive ability of domestic tomato products determines the irrational relationship between price and quality of the manufactured articles. To increase the quality of canning output and to reduce its prime cost is possible due to the thorough selection of the types of tomatoes and use of highly technological production processes. In the scientific arsenal of the technologists of  Daghestan State Technical University and Kuban State University of Technology has already been located high technologies and pour on the mechanisms of action on the qualitative composition of the tomato raw material of multi-frequency electromagnetic and ultrasonic. By the large potential of highly technological working of raw material possess the methods of the preparative separation of the components of raw material the liquified and compressed gases. New technological methods make it possible to produce production without the preservatives, the fillers, the chemical, undesirable aromatic substances and other additives. In this work we propose effective means of the solution of this problem in the use of physical, physical chemistry and chemical methods of action on the tomato raw material, semifinished products, auxiliary materials and finished production. They include the electromagnetic field of low and superhigh frequencies, ultrasound, the SO2- working, utilized in other branches industries.

SPOROBOLUS CRYPTANDRUS (TORR.) A. GRAY (GRAMINEAE) – INVASIVE SPECIES OF FLORA IN THE ROSTOV REGION O.N. Demina, L.L. Rogal, S.R. Mayorov   In the Rostov region (Russia), in the sand in the river valleys of the Seversky Donets, Derkul and Kalitva it was discovered Sporobolus cryptandrus (Torr) A. Gray. – North American adventive species. In recent years, recorded an increase in its population, which is related to the increase of anthropogenic load, including the resumption of active use of these territories as the grazed areas. At the same time, the fight against the settling of alien species that may subsequently become dangerous quarantine weeds, more cost-effective in the early stages of infestation. In view of this, an urgent need to pay close attention to the study of the biology and ecology of  S. cryptandrus. In the near future it is important to carry out an assessment of limiting fact affecting its size and the speed of the processes of invasion species.

The influence of SO2 on the plate of the leaves of planted trees In the article the influence of SO2 on the morphometric indicator of leaf plates of some planted trees has been researched. According to the gathered information the morphometric indicator of leaf plates and its connection with the concentration of sulphur in the atmosphere have been envisioned.

Role of inhalation of Abisib (water extract of Abies sibirica needles) in correcting the functional characteristics and productivity of broiler chickens in intensive poultry farms. Our studies have shown that the use of Abisiba (water extract of Abies sibirica needles) elevates erythrocyte count in peripheral blood in the experimental group of chickens. Average live weight in the experimental group (by 7%) and higher survival rate (by 6.5%), per cent concerning the control group that certainly shows the effectiveness using of Abisib in condition of intensive rearing of broiler - chickens.

The daily and seasonal activity of South Siberian brown bear (Ursus arctos baicalensis) in the area of artificial feeding in the Baikal region The article summarizes the results of hunting industry monitoring of brown bears (Ursus arctos baicalensis) (Litvinov, 2000) with automatic surveillance camera at the experimental site of training experimental hunting unit  "Goloustnoye" of the game management faculty of Irkutsk State Agrarian University in the period from 2013 to 2015. Six hundred and seventy - five data files were analyzed. It was found that the activity of bears in the areas of observation is directly related to the time of day and season of the year. There are relationships between the behavior of the animals and the air temperature.

Photobacterium phosphoreum as an object for studying the biological effects of the CeO2 nanoparticles Gretsky Igor Alexandrovich, Zholobak Nadezhda Mikchailovna, Scherbakov Alexander Borysovich, Ivanov Vladimir Konstantinovich, Gromozova Elena Nikolaevna Abstract: The effect of nanoceria (CND), that have different physical and chemical properties – the used producing methods, Zeta potential, the presence of the stabilizer – on the the intensity of luminescence of Photobacterium phosphoreum IMV B-7071 was studied. It is shown that the high crystallinity nanoceria after 30 minutes after addition stimulated the cells luminescence, whereas the effects of citrate-stabilized CND depend essentially on the toxic effects the stabilizer sodium citrate.

Agrogene Transformation and Humus State of Soil in Farmland of Orenburg Zauralye Сhernozem southern and dark chestnut soils in various farmland (virgin land, hayfield, grazing areas, fallow land, arable land) are investigated in Orenburg Zauralye. It was established that the agrogene transformation of ecosystems is associated with the following processes: 1) decrease of phytocenosis productivity on account of reducing underground biomass; 2) deterioration in the structural-aggregate state of the soils; 3) easing of the intensity of carbon dioxide emission from soils; 4) reduction of humus content (for 8% -29% of the original value); 5) changes in fractional-group composition of humus in the direction of reducing the content of GA2 fraction.

New geological and geochemical data of prospects of oil-and-gas content of deep-laying deposits of the North of Western Siberia Geological structure of deep-seated deposits in the north of Western Siberia, lithology and reservoir properties of the Jurassic and pre-Jurassic rocks, geochemical characteristics razreza were refined. Proizvedena preliminary assessment of hydrocarbon potential has made. The dependence of the quantities of hydrocarbon reserves on the deposits from paleofatsialnoy depositional environment, sediment thickness and catagenetic transformation of the original organic matter.