Efforts to preserve natural and human habitats through environmental conservation aimed at modifying their metrics always been made over the years. The goal of this research is to quantify changes in ecosystem indicators in Con Cuong district, Nghe An province, Vietnam from 2000 to 2015. This research area also has Pu Mat national park where the biodiversity-rich in habitat, species, genes as well as ecosystems. In this article, seven indicators are measured with GIS and Fragstats Software, including Shannon's Diversity Index (SHDI), Simpson's Diversity Index (SIDI), Number of Patches (NP), Patch Density (PD), Percentage of Like Adjacencies (PLADJ), Interspersion and Juxtaposition Index (IJI) and Edge Density (ED). The value for each indicator will be assigned for each observation to capture the character of the ecosystem. They will be compared with each other and considered changes in ecosystems of the forest, agricultural, artificial and others. The method of thematic overlays and transition matrixes has also been used to provide precise identification of the ecosystem changes that have taken place over the period under review. A detailed explanation and mapping of the dynamics of the ecosystem was obtained as a consequence. This work can be replicated and evolved into a methodological phase to show the indication of the ecosystem, because it adds a reference value for the study of differences in ecosystem characteristics. The findings show that the difference in the environment is mostly due to the decrease of naturalness and the increase in the complexity of agriculture ecosystems and urban growth.