1.5.19. Почвоведение

The features of the metabolic processes of transformation of the organic and mineral components of the soil are determined mainly by the complex of soil enzymes, the abundance and qualitative composition of the microbiocenosis. The activity of soil enzymes is an additional diagnostic indicator of soil fertility and its changes under anthropogenic impact. Studies of the activity of soil enzymes in various zones of the city of Sumgait - a large industrial center in which the petrochemical and oil refining industries are concentrated. The results of field studies showed that the activity of soil enzymes was not the same in different zones of the city of Sumgait. Significant differences in the catalase activity of soils of roadside and industrial zones and soils under parks have been established. With technogenic pollution, the functional activity of soil microorganisms is significantly reduced. In comparative terms, the activity of all soil enzymes is most suppressed in industrial zones, and to the least extent in park zones. The analysis shows that the technologically polluted soils of the industrial city of Sumgayit are characterized by weak stability of soil enzymes. These indicators reflect the specificity of pollutants, the degree of contamination, the nature and intensity of biological processes in soil affected by industrial impacts - industrial and transport emissions. The research results can be part of the database for an objective assessment of the soil cover of urban landscapes in Sumgait, to prevent the deterioration of their environmental functions, for the development of the scientific basis for the use of microorganisms in technologies for cleaning and increasing the fertility of urban soils.  

The aim of the study is a comparative analysis of the results of determining the particle-size distribution of soils obtained by various methods: the field wet method, the pipette method, and also regulated by international (ISO 11277: 2009) and interstate (GOST 12536-2014) standards. Investigations of the particle-size distribution for ordinary calcareous chernozems have shown that the causes of inconsistencies in the analyzes results of the same soil samples using the pipette-method and the areometric method according to GOST 12536–2014 and ISO 1127762009 are exclusively associated with different approaches to preparing soil samples for laboratory tests. First of all, the analyses data are depended on the ratio of the soil sample mass and the dispersant volume.  

The article is devoted to Pavel Alexandrovich Sadimenko, whose 100th anniversary was celebrated in July this year. A prominent soil scientist whose scientific interests were concentrated in the field of land reclamation and soil physics, Professor P. A. Sadimenko also paid great attention to the study of the trace element composition of soils.

The yield of soybean varieties Kazachka erosion-dangerous slope depending on the method of basic soil treatment and mineral fertilizers Rychkova M. I. Mishchenko A.V. Federal Rostov agricultural research center, Rassvet village, Russia, E-mail: rychkova-1980@list.ru Annotation: The results of three-year field studies on the influence of methods of basic tillage and mineral fertilizers on the yield of soybean varieties Kazachka, obtained in the conditions of erosion-dangerous slope of ordinary carbonate chernozems (according  WRB, 2014, – Haplic Chernozem) of the Rostov region. Studies have found that in the conditions of erosion-dangerous slope an effective way of basic soil treatment in the cultivation of soybean varieties Kazachka is chisel and the introduction of an increased level of mineral fertilizers norm N84P30K48 (162 kg d. V. per 1 ha of crop area). At the same time, the highest indicators of productive moisture reserves in the soil and yield increase are formed-0.38 t/ha. Chiseling contributed to an increase in the payback of 1 kg of fertilizers by an increase in yield by 0.13-0.35 kg/kg in comparison with plowing.

Architectonics of the kidneys in connection with frost resistance Kidneys, like seeds, are of great importance in preserving plant life, especially in adverse environmental conditions. The structure of the kidneys consists of tissues and parts with varying degrees of thermal conductivity. A method for assessing the resistance of kidney to frost is proposed, depending on the internal structure of structures and location in space.

Entry of Zn and Cd from soil into herbaceous plants of the Asteraceae and Poaceae families Abstract: Environmental heavy metals pollution is one of the most pressing problems of nowaday ecology. Heavy metals are not subject to biodegradation and tend to move along the food chain, thereby threatening the animals and humans health. The aim of the work was to study Cd and Zn accumulation in different wild-growing herbaceous plants species of the Asteraceae and Poaceae families under long-term technogenic pollution. Ecological monitoring of the impact zone of the city of Novocherkassk, subjected to many years of technogenic pollution, was carried out. In plants growing in a 5 km zone around Novocherkasskaya power station, contamination with the studied elements was revealed. The soils of monitoring sites located in the prevailing north-west direction of winds are subject to the greatest pollution. The dependence of the content of Zn and Cd in the herbaceous plants of the Asteraceae and Poaceae families on the distance to the source of the technogenic load was established. The selectivity of metal accumulation by the studied species of herbaceous plants at various levels of technogenic pollution was revealed. Plants of the Asteraceae family accumulate the studied elements mainly in the aboveground part, Poaceae are characterized by a greater accumulation of metals in the root system. The least resistant to the input of pollutants from the soil is the Achillea nobilis. Poa pratensis has the highest resistance to Zn and Cd pollution by the combination of assessment indicators. Cd has a higher mobility in soils than Zn, which leads to a higher level of plant pollution with this element. The particle size distribution of the soil also has a significant effect on the availability of metals to plants.

The theoretical basis for the use of humic preparations on irrigated light serozems The considered materials testify that the development of the questions of the biochemistry of humus and humus substances, the use of biohumus in agriculture of irrigated light serozems lags behind the general state of this section of soil science. Studies of humus in soils and biohumus will provide additional opportunities for theoretical and practical developments that allow you to solve the addition and the necessary issues of agricultural practice in the region.

Immunological phenomenon of physical antigen-specific interaction of mononuclear cells with granulocytes in vitro Evstropov Vladimir M. Don state technical University, Rostov-on-Don, Russia Abstract:  Currently, only some aspects of the physical contact antigen-specific interaction of lymphocytes with granulocytes have been described (5), but meanwhile it is known that not only various subpopulations of lymphocytes, but also macrophages play a leading role in the formation of specific (adaptive) immunity. In recent years, neutrophils have also become regarded as cells involved in the recognition of a pathogen; therefore, the physical interaction of these cells, which are excorded from the immune system, in the presence of a causally significant antigen in vitro is of undoubted interest. According to some researchers, to study a specific cellular immune response as a criterion for assessing the effectiveness of immunization, it is advisable to use laboratory methods in vitro using specific antigens [7]. The aim of this work was to study the feasibility of assessing the indication of specific immunity in vitro by characterizing the physical antigen-specific interaction of mononuclear cells with granulocytes isolated from rat blood immunized with staphylococcal disintegrant in the presence of incomplete Freund's adjuvant. In this case, the quantitative level of rosettingformation mononuclear cells interacting with three or more granulocytes in vitro in the presence of this antigen was evaluated. The results of the study showed an increase in the quantitative level of rosetting mononuclear cells, compared with the same indicator of non-immune rats of the control group. The paper considers the possible participation in the implementation of this immunological phenomenon of subpopulations of peripheral blood T-lymphocytes (naive T cells, CD45RA-positive effector cells, antigen-reactive T cells), as well as NK cells, monocytes and neutrophils. It is assumed that this method can be used to detect an immune response, however, the molecular mechanism of this immunological phenomenon and its immunological classification (attribution to the cellular or cell-mediated type of reaction involving cell-fixed antibodies) needs further study.

On the biological degradation of white phosphorus On the biological degradation of white phosphorus   1 Institute of Organic and Physical Chemistry named after A.E. Arbuzov. Kazan Scientific Center of the Russian Academy of Sciences. Arbuzov St., 8. Kazan, 420088. Republic of Tatarstan. Russia. E-mail: mindubaev-az@yandex.ru 2 Kazan (Volga Region) Federal University. University St., 18. Kazan, 420008. Republic of Tatarstan. Russia. Abstract For the first time different taxonomic groups of microorganisms (fungi, Streptomyces and bacteria) are inoculated on culture medium containing white phosphorus as the single source of phosphorus. On these media microorganisms grew and have not experienced phosphorus starvation. It is the world's first example of the inclusion of white phosphorus in the biosphere cycle of elemental phosphorus. The highest concentration corresponds to 5000 times excess of MPC of white phosphorus in wastewater. The comparison of the sequences of ribosomal genes of the fungus, steadily metabolizing the white phosphorus, with sequences of the GenBank database, allowed us to identify this microorganism as a new strain of Aspergillus niger, to which we have assigned the number A. niger AM1. Inoculation of A. niger AM1 in medium containing just two sources of phosphorus (phosphate and white phosphorus) demonstrated that P4 does not exhibit toxic properties in relation to this microorganism. In the presence of white phosphorus it grows at the same rate as in the absence thereof. This is the only example of the lack of white phosphorus toxicity to a living organism. Our previous studies have demonstrated the absence of white phosphorus toxicity for Aspergillus niger AM1. However, the toxic properties of the substances are of different nature. It is of great interest to study the genotoxicity – a possible source of mutations. In the present work SOS-lux test has demonstrated genotoxicity of white phosphorus. This result is obtained for the first time – all the available literature sources reported no genotoxic properties of white phosphorus. Allium test showed the mitotoxic effect of white phosphorus on eukaryotic cells. A very interesting and unexpected result was the discovery of a mutant Aspergillus niger AM1. In comparison with an ancestral strain this mutant possesses an unusual morphology and grows more intensively in culture medium with white phosphorus. Three strains of A. niger, sent from the All-Russian collection of microorganisms, also showed much higher resistance to white phosphorus than bacteria. According to preliminary data, resistance to white phosphorus the A. niger is fixed in the genome.

Resource-saving elements of soybean cultivation technology on the slopes of the Rostov region Resource-saving elements of soybean cultivation technology on the slopes of the Rostov region Rychkova M.I., Gaeva E.A., Mishchenko A.V. Federal Rostov Agricultural Research Center, Rassvet. Е-mail: emmaksay@inbox.ru. Abstract. The article presents the actual results of research on the development of resource-saving elements of soybean cultivation technology, providing moisture saving, high productivity and soil protection from erosion. Two-year studies were carried out on the erosion-dangerous slope of the South-Eastern exposure of the Bolshoy Log beam, Aksai district of the Rostov region in the system of contour-landscape organization of the territory in 2017-2018. Studied two methods of primary tillage – otvalnuyu adopted for monitoring and chisel. Chisel tillage in the cultivation of soybean was performed by chisel plow. Dump processing was carried out with a plow blade. The mineral nutrition system included three backgrounds: natural fertility recommended by zonal systems and increased by half. It is established that to prevent possible erosion it is recommended to cultivate soybeans in the system of contour-band placement of fields. It is shown that the payback of the crop fertilizers on the version with the chisel treatment of the soil is higher than on the dump. Increasing the dose of fertilizer in half reduces the payback of fertilizers by 18.9-19.4 %. It is revealed that the approved soil protection (chisel) tillage can significantly reduce the erosion processes – soil flushing by 22.9%, runoff – 10.0%, the cost of fuel and lubricants-18.8%, increase the accumulation of reserves of productive moisture in the soil with a more economical expenditure, yield – by 30.2 – 32.1%, conditional net income-up to 6.6 thousand rubles /ha, profitability-by 42.1%, recoupment of direct costs yield of 1.4 rubles per ruble in comparison with the same indicators at the dump plowing.

Location on the Caucasus Potentilla multi-advisable (Potentilla multifida L.) Location on the Caucasus Potentilla multi-advisable (Potentilla multifida L.)  O.N. Demina, L.L. Rogal'  Abstract: The paper describes the Gum-Bashi Pass, which is named after the homonymous right-bank tributary of the Kuban - the Mara River. The distribution of grass vegetation on the Gum-Bashi Pass, as on the whole Rocky Caucasus Mountains, is associated with vertical zonality or altitudinal belt ecosystems. The characteristic of indigenous subalpine vegetation is given: on gentle northern slopes and plateaus of the Rocky ridge formed lush grassy meadows; Petrophytic steppes develop on the steep slopes of the southern, southeastern, and southwestern exposures. The most common types of natural forage lands on the Gum-Bashi Pass are mountain meadow communities on mountain sod-podzolic, gray and brown forest soils, and meadow-steppe communities on leached and podzolized black soil. However, the main purpose of the work is to describe a new plant species, Potentilla multifida L., which we discovered for the first time in the territory of Karachay-Cherkessia, in the Rocky Ridge system on the Gum-Bashi Pass and confirming its growth in the Caucasus. In describing this species, it is mentioned that this herbaceous perennial reaches 40 cm in height. The leaves of the plant are green above and beloiclochic below, dissected into linear lobes. The flowers are small, on thin pedicels, pale yellow. The beginning of its flowering was recorded when the species was collected. A critical summary of references to this species and an indication of the need to describe a new Caucasian form is given. In conclusion, one more time focuses on the need for this description, studying the distribution of P. multifida in the Caucasus and its phytocenotic environment. The article is illustrated with a large number of photos.

Effect of different phytohormones on Austrian sage multiplication (Salvia austriaca Jacq.) Effect of different phytohormones on Austrian sage multiplication (Salvia austriaca Jacq.) Vasilchenko Elena V., Sereda Mikhail M., Varduni Tatiana V. Southern Federal University, Rostov-on-Don, Russia  Abstract: The influence of phytohormones such as 6-benzylaminopurine, naphthylacetic acid and indole-3-acetic acid on the Austrian sage multiplication (Salvia austriaca Jacq.) in an in vitro culture is discussed in the paper. Austrian sage is the medicinal, essential oil, melliferous and ornamental steppe herb, included in the Red List of the Rostov region. The study was conducted in the laboratory of cellular and genomic technologies of plants of the Botanical Garden. Seeds from the collection of the Botanical Garden of the Southern Federal University were used as the primary explant. For cultivation the Murashige and Skoog medium was used. Murashige and Skoog medium without the addition of phytohormones was used as a control. Evaluation of the multiplication efficiency was carried out after 21 days. The multiplication factor was calculated as the number of new shoots. The highest multiplication factor was observed in the medium with the addition of 0.5 mg / l of 6-benzylaminopurine and 0.1 mg / l of naphthylacetic acid. This concentration of phytohormones is recommended for micropropagation of Austrian sage. The concentration of auxins (IAA and NAA) 0.3 mg/l leads to a decrease in the efficiency of Austrian sage multiplication. The height of shoot was approximately the same and no statistically significant differences were found on all tested mediums. It should be pointed out that with a greater concentration of phytohormones physiological abnormalities was observed.

Methods of soil fertility preservation on eroded slopes of Rostov region Methods of soil fertility preservation on eroded slopes of Rostov region Gaevaia Е.А. Federal Rostov Agricultural Research Center, Rassvet. Е-mail: emmaksay@inbox.ru. Abstrac: Тhe Studies were carried out in a multifactorial stationary experiment in 1991-2018 years. the Experiment is located on the slope of the beam of the Big Log of Aksai district of Rostov region. The soil of the experimental site is ordinary heavy loamy Chernozem on loess-like loam, medium-eroded. In the experiment studied two crop rotation zernopererabotki (I) and sertralineprograms (II), and three levels of organic-mineral system of fertilizers («0» – natural fertility; «1» – cattle manure 5 t + N46P24K30 and «2» – cattle manure 8 t + N84P30K48 1 hectare). The aim of the study was to study the balance of humus depending on the design of crop rotations located on the eroded slopes of the Rostov region, to identify ways to preserve soil fertility at the initial level and increase it. As a result of the research, the amount of washed soil was determined. The importance of plant residues in the accumulation of humus is shown. It is established that the intensive use of agricultural land in the Rostov region without fertilizer leads to a reduction in the humus content in the soil. The role of perennial grasses in reducing soil flushing by 35.6% was studied. It is shown that the long-term use of organic fertilizers in crop rotations at a dose of 5 tons of cattle manure and N46P24K30, as well as the introduction of 20% of perennial in the crop rotation allows to maintain a positive balance of humus at the level of (+35 kg/ha) in the grain-grass crop rotation. Increasing the dose of fertilizer to 8 tons of cattle manure and N84P30K48 contributes to the accumulation of humus up to (+100 kg/ha) in the grain crop rotation. The possibility of replacing organic fertilizers with 1-2 t/ha of wheat straw using compensatory doses of nitrogen fertilizers is shown.

To the future without poliomyelitis: the state of the epidemic, vaccination issues (review) To the future without poliomyelitis: the state of the epidemic, vaccination issues (review) Belova Olga Evgen’evna  FSRI SRC VB «Vector», Koltsovo, Novosibirsk reg., Russia  Abstract: Polio is a highly contagious viral infection that affects mainly children under five years of age, which can lead to paralysis or even death. Damage to the nervous system is manifested by fever, pain in the limbs, then peripheral irreversible paralysis. In severe cases, paralysis of the respiratory muscles can occur, which causes death. The transmission mechanism is predominantly fecal-oral. The only way to combat polio remains vaccination. The vaccine provides the development of long-term humoral and local immunity. The purpose of this work is to analyze the epidemiological situation of poliomyelitis in the world 31 years after the adoption of the Global Polio Eradication Program, and to compare the characteristics of live and inactivated polio vaccines. Issues related to the use of live oral polio vaccine and the need for its phased withdrawal from circulation, the problem of vaccine-related polioviruses and vaccine-associated paralytic polio are considered. Describes ongoing research into the development of new safe and effective polio vaccines; approaches to the development of new IPV to reduce costs, increase coverage and reduce the risks of using live polioviruses in vaccine production; new technologies for the delivery and administration of IPV; clinical studies of new vaccines and vaccination schedules. The main objectives of the strategy to complete the eradication of poliomyelitis for 2019-2023 are discussed: eradication, integration, certification and containment.

The ameliorative condition of the soil spread around the Kattakurgan reservoir and its changes The ameliorative condition of the soil spread around the Kattakurgan reservoir and its changes Abdullaev S.A.1, Jabbarov Z.A.1, Tursunulova A.1, Okolelova A.A.2, Kholdorov Sh.M.3 1National University of Uzbekistan, Tashkent, Uzbekistan, zafarjonjabbarov@gmail.com 2Volgograd State Technical University, Volgograd, Russia, allaokol@mail.ru 3Tokyo University of Agriculture and Technology – TUAT, Tokyo, Japan, sh.m.xoldorov@gmail.com Abstract: Changes in the physicomechanical properties and ameliorative state of the soil were studied using the Kattakurgan reservoir as an example; the degree of fossil, the density of irrigated lands, the dynamics of the groundwater level by years, the ameliorative state and the location of salts by layers were studied. According to the results, the indicator of soils with an inadequate ameliorative state increased from 4.22% to 5.58%. It was justified that the level of groundwater salinity is mainly between 0-1 g / l and 1-3 g / l. It is scientifically substantiated that the main effect of the Kattakurgan reservoir on soils is formed by the accumulation of salts, the effect on the physicomechanical properties is relatively small.

Study of the influence of parkland of the Botanical Garden of the Southern Federal University on the parameters of the microclimate Study of the influence of parkland of the Botanical Garden of the Southern Federal University on the parameters of the microclimate A. S. Bezrukova, B. L. Kozlovskij, M. V. Kuropyatnikov Southern Federal University (SFedU), Rostov-on-Don Abstract: Rostov region refers to areas with uncomfortable conditions. Therefore, to create a comfortable environment requires special events. In cities, forest parks, parks, gardens and squares create a favorable microclimate. Analysis of the literature shows that the influence of green spaces on the microclimate of southern cities is not well studied. The aim of the work was to assess the impact of the park of the Botanical Garden of the Southern Federal University on the microclimate. As the object of the study were taken different in species composition plots of the park of the Botanical Garden of SFedU. As part of the study, measurements were made of the temperature and humidity of the air using the UNI-T UT333 device at a height of 1,3 m from the soil surface, as well as the surface temperature using the UNI-T UT320D device. The measurements were carried out on pedestrian alleys and paths. In the course of the research, it was established that the park stands effectively affect the microclimate parameters (air and surface temperature, air humidity), changing them in the direction of more comfortable. It is proved that there is a reliable difference between the values of the measured parameters at open space and in the park area with a confidence level of 99,9 %. The greatest effect of changes in air temperature and road network surfaces is observed at noon, then it decreases. The absolute difference in humidity between the park and open spaces during the day does not change. The ameliorative effect of parkland on of surrounding areas is spread weakly. The degree of change of microclimate parameters does not depend on the species composition of woody plants growing on it.  

Catalogue of the rare and extinct plant's collection of the Rostov region in the Botanical garden of the Southern Federal University Catalogue of the rare and extinct plant's collection of the Rostov region in the Botanical garden of the Southern Federal University Shmaraeva Antonina N., Shishlova Zhanna N., Kuzmenko Inna P., Firsova Anna V. Botanical garden of SFU, Rostov-on-Don, Russia; anshmaraeva@sfedu.ru Abstract: The Botanical garden of Southern Federal University (BS) for more than 90 years of its existence turned into the center of the conservation of biodiversity of plants. From the moment of its creation the methods of an introduction of plants in a steppe zone were developed in the Botanical garden, woody and grassy plant's collections were formed. The BS modern collection fund is presented by 17 thematic collections and expositions of the outdoor and in closed greenhouses. One of priority problems of BS is preservation of rare species of regional flora ex situ, that is: in the collections and expositions. To these targets serve, first of all, the collection of rare and endangered species of plants of the Rostov region. When forming a collection the new knowledge and practical material received in the course of implementation of long-term projects on maintaining the Red List of the Rostov region are used. Now the collection of rare and extinct plants of the Rostov region contains 75 species of flowering plants (or 40% of total number of the protected seed plants of the Rostov region) from 28 families and 55 genuses, including 24 species included in the Red List of the Russian Federation and 6 species included in the Annex to the Red List of the Russian Federation. The grassy perennials, xerophytes, species of steppe ecology prevail in the collection; and in the geographical relation – the species, connected with the arid centers of origin. The species with category of a rarity 3 (rare), and category of a rarity 2 (reducing in number). are presented in the collection in more or less equal degree. Six species have category of a rarity 1 (being under the threat of extinction) and four species – category of a rarity 5 (restored).  

The change of absorbed bases composition in desert soils as an indicator of their evolution  The change of absorbed bases composition in desert soils as an indicator of their evolutionYuldashev G.1, Isagaliev M.T.1, Bezuglova O.S.2 1Fergana State University, Republic of Uzbekistan 2 Southern Federal University, Rostov-on-Don, Russia   The research is aimed to clarify the role of the capacity of the soil absorbing complex and the composition of cations in the evolutionary transformations of the irrigated desert soils. The object of research comprised the soils of large regions of oasis agriculture of the lower reaches of the Amu Darya and Central Fergana, where irrigated meadow, floodplain alluvial and irrigated meadow, oasis soils are distributed respectively. Considering the alluvial nature of the deposits on which the studied soils are formed, they can be attributed to the reference soil group Fluvisols (Fluvisols). Absorbed bases were determined by the Pfeffer method in Kruger modification. It was established that the studied soils are characterized by certain values ??of the absorption capacity and the ratio of cations in the composition of the soil absorbing complex, depending on the duration of irrigation and a combination of soil formation factors. At the same time, the average content of each cation in the composition of the soil absorbing complex is relatively stable for the soil. In the process of evolution, which occurs under the influence of agrotechnical and agromeliorative methods of using the soil, these indicators change slowly following the change in the grain size distribution. In general, the amount of absorbed bases increases with the increase in the cultivation of irrigated hydromorphic desert soils. The chemical composition of alluvial and alluvial-proluvial pedogenesis soils also influences the characteristics of soil formation in the desert zone and the specifics of their ameliorative characteristics. According to the content of Ca ++, Mg ++, K +, Na + as part of the soil absorbing complex, the potential capabilities of the soils and the efficiency of land reclamation are determined, measures are being taken to increase the fertility of irrigated soils.

The paper aims to assess the impact of urbanization on the quantitative characteristics and species composition of microbial communities of the urban soils of Rostov-on-Don. 10 soil samples characterized by a dramatically different level of anthropogenic impact and transformation have been studied. The microbial communities of the upper horizons of weakly disturbed natural soils (Haplic chernozem) were compared to those from the urbic horizons of the Technosols in the central part of the city. It has been shown that the process of urbanization leads to a fundamental change in the microbial communities of urban soils in the steppe zone. There is a decrease in species diversity in the microbial communities. In addition, in anthropogenically transformed soils there is an increased number of copiotrophic bacteria compared to weakly disturbed soils. The number of aerobic spore-forming bacteria of the order Bacillales decreases several times. The species composition also changes, which is manifested in the disappearance of typical species from microbial complexes of anthropogenically transformed soils. The proportion of rapidly growing species of bacteria (r-strategists) is increased in the microbial communities of urban soils, indicating that the soils are under stress. The stability of the soil ecosystem is impaired and this may lead to the loss of its ecological functions. In addition, an increase in the proportion of microorganisms with antioxidant pigments was observed in anthropogenically transformed soils.

Rare species of plants and fungi in the territories of protected natural objects of the Rostov region Rare species of plants and fungi in the territories of protected natural objects of the Rostov region Fedyaeva Valentina Vasilyevna1, Shmarayeva Antonina Nikolayevna1, Hibukhina Tatyana Yuryevna2, Shishlova Zhanna of Nikolayevna1, Kuzmenko Inna Petrovna1 1 Southern Federal University, Rostov-on-Don, Russia; vfedyaeva@gmail.com 2 Ministry of Natural Resources and Environmental Protection of the Rostov region, Rostov-on-Don, Russia   The formation of network of the especially protected natural territories (EPNT) is one of the most effective mechanisms of preservation of the natural complexes and objects in World practice, including biological diversity of plants. 82 EPNT with a total area of 233.49 thousand hectares operate in the Rostov region now, covering 2.31 % of its territory. It is much less than average World's level (8.8 %) and an than average Russia's level (13.6 %). In the course of the researches the biological diversity of plants and fungi was defined on EPNT of status category: «protected natural objects», including the species, engaged in the Red List of the Rostov region. The protected natural objects are the unique single natural objects and unique territories, having the area less than 100.0 hectares, representing scientific, educational, cultural and esthetic value. The protected natural objects are presented in 13 of 43 administrative regions of the Rostov region. The total area of the protected natural objects which are located in different parts of the area to the date 01.03.2018 is 1.07 thousand hectares which corresponds to 0.50 % of total area of EPNT, operating in the Rostov region. In recent years it was established, that in the territories of protected natural objects of the Rostov area 98 species of plants and fungi occur, which are included in the Red List of the Rostov region (35.9 % of the total number), including 26 species engaged in the Red List of the Russian Federation – 51.0 % of the total number of the «Red List» federal objects, growing in the territory of the Rostov region.