14.03.06.Фармакология, клиническая фармакология

The epidemiological situation of West Nile fever in Volgograd region West Nile virus for the Volgograd region is the most important arbovirus infection. The observed decrease in the incidence of WNV from 2001 to 2006, in 2008 and 2009 years, reducing the number of severe disease is only indicative of a temporary regression of the epidemic process in the Volgograd region. In the Volgograd region there are prerequisites and precursors complications epidemiology of West Nile fever. The purpose held in the Volgograd region surveillance in West Nile is to provide the information necessary and sufficient for the analysis of the epidemiological situation, as well as to determine the direction of policy and action in public health management decisions.

Molecular detection of baculoviruses of beet webworm from the collection of All-Russian Institute for Plant Protection Two isolates of baculoviruses from beet webworm Loxostege sticticalis (Lepidoptera: Pyraloidea) deposited in the collection of entomopathogenic organisms as a granulovirus (GVLS-85) and a nucleopolyhedrovirus (NPVLS-90) have been subjected to genotyping using degenerate primers L8F2:L8R2 flanking a conservative region of late elongation factor gene lef8. Both isolates, tested independently to avoid sample cross-contamination, were found to belong to a single molecular haplotype. This haplotype is unique, showing maximum sequence similarity of 73% to Pieris rapae granulovirus (Genbank accession number JX968491) and other granuloviruses. No significant similarity to available sequences of nucleopolyhedroviruses have been found.

Detection, identification and quantitation of bocavirus among children with acute respiratory viral infections and gastroenteritises in Moscow Human bocavirus (HBoV)) causes acute respiratory virus infections and gastroenteritises in humans throughout the world. HBoV DNA detection and identification among children with respiratory infections in Moscow during 2005–2010 by means of reverse transcription with subsequent real time PCR with fluorescent probes showed the long-term average frequency 14, 5 ± 0, 7% for 2, 395 nasopharyngeal lavages (variations in a rang from 13, 5% до 17, 0%). One should note seasonal activity of the HBoV circulation during a year with 2 peaks in autumn-winter and early spring. Among infants and toddlers less than 1 year old the HBoV was associated with other respiratory viruses, whereas for children 2–7 years old the bocaviral monoinfection was prevailed. Gastroenteritises were diagnozed in 34, 3 ± 3, 7% clinical cases. The HBoV viral loads in 40, 5% samples were more than 105 genome-equivalents per 1 ml for the first days postinfection, whereas for mixed respiratory infections and for children with the HBoV monoinfection were less 103 genome-equivalents per 1 ml.

Studying cyto-anatomical changes in tissues of virus infected Solanum tuberosum and Nicotiana tabacum plants Virus impacts on affected by them potato plants appear in stunting, the plant cells deformation and changing their structure. In virus-affected potato tubers, reduces quantity and size of starch grains. Obtained virus-containing callus from the test-plants consist of elongated and malformed cells containing amorphous inclusions.

Analysis of the tick-borne encephalitis virus coding sequences We have conducted an analysis of tick-born encephalitis virus (TBEV) coding sequences that are available via international data bank (GenBank). Upon comparison of polyprotein structures of 113 TBEV strains from various sites from Europe and Asia we have detected genotype-specific combinations of aminoacid substitutions. It was shown that strains Buzuuchuk from Kyrgyzstan and 178–79 from Russia (Eastern Siberia) have unique genomic structures.

Orthobuniaviruses as main factor for emergency diseases This analytical paper deals with the systematic and taxonomy of orthobuniaviruses, small developed dangerous and new viruses Akabane, Aino, Schmallenberg, Cache Valley diseases, Oropouche fever, Iquitos. The significance of reassortment mechanism of their origin and diversification are discussed.

Spreading of virus diseases of fruit-trees and small fruit cultures and modern techniques for their sanitation The spreading of different viruses on varieties and rootstocks of pome fruit trees, stone fruit plants, and small fruit cultures has been studied. It was established that efficiency of sanitation technique from viruses depended on culture, virus species and therapy method. The best results for obtaining virus free material were achieved by means of complex application of in vitro chemotherapy and magnetotherapy.

Preservation of infectious activity of industrial strains of rabies virus stored at different temperatures The dependence of specific infectious activity on the cell culture model of rabies virus industrial (fixed) strains L. Pasteur and CVS from the duration and storage temperature was investigated. Viral suspension was produced by cell culture BHK-21 / C13 and frozen at -20 and -80°C. Analysis of the specific activity was performed after 1 week, 1, 2, 3, 6 and 12 months of storage. It was experimentally discovered that the temperature -80°C is more effective for long-term storage for the studied strains.

Lithium salt of acrydon-acetic acid as a substance for biomedical research The article considers a new antiviral and imunomodulating drug with extremely high bactericidal, and particularly virucidal activity against RNA and DNA viruses, mycocidal and algicidal effect. Ways of the effective use of the drug in the national economic products are discussed.

The hybrid forecasting model on the basis of intellectual agents The hybrid model on the basis of intellectual agents is developed for the task of infectious dynamics prediction. Intellectual agents are created as recurrent neural networks and interact on the basis of the Markov chain. In comparison with traditional mathematical models the hybrid forecasting model of infectious dynamics has following advantages: take into account epidemic’s numerous parameters, possibility of model’s scaling and setup for different types of epidemics.

Тhe frequency of the human papillomavirus in the population of the Rostov-on-Don In this study the detection frequency of human papilloma virus types with high carcinogenic risk among residents of Rostov-on-Don was analyzed. The frequency of HPV-positive samples among women was 40%, among men — 21, 5%. Detection frequency of human papillomavirus was highest among young persons up to 35 years. More than 40% of HPV-positive persons had an average concentration of virus which may cause the cell dysplasia.

Virus inhibitory effect of the complex interferon inducer of type I in vivo in experimental herpes infection Ativiral effect of the molecular composition (MC) yeast RNA-tilorone and 2, 7-bis[2-(diethylamino)ethoxy]-fluoren-9-on dihydrochloride (tilorone) was demonstrated on the experimental model of herpes infection in vivo. The reduction of mortality by 80 % was observed in the group of herpes-infected animals treated with the MC, and the average life expectancy increased by 1, 5 times compared to the control. A reduction of the level of herpes simplex virus replication in the infected animal brain tissue was identified as the result of MC activity. The molecular composition of the yeast RNA and tilorone showed the most significant anti-herpetic effect comparing to the officinal drugs — Virolex and рoly(І)-poly(C).

The founder of the doctrine of viruses (To the 150th anniversary of the birthday of D. I. Ivanovsky) Describes the main stages of scientific and pedagogical activity of the D. I. Ivanovsky. Main results of his research, which determine the undisputed priority of the scientist as the founder of the doctrine of viruses.

Study of the stability of collection of potato varieties to virus diseases in the forest-steppe of Ukraine Studied 630 potato varieties for resistance to the major viral diseases in the forest-steppe of Ukraine. Established that the visual assessment of diseases complicated by nonspecific symptoms of some viral diseases. Using immunological and electron microscopic methods were identified pathogens of viral infections — Potato virus M and Potato virus Y. Highlighted accessions resistant to potato leaf roll, leafdrop streak and wrinkled mosaic.

On a plant Phryma asiatica (Hara) Probat. for the first time is revealed the isometric virus with the following characteristics: diameter virions — 30–32 nanometers; molecular weight of a capsid protein — 41,4 ± 0,8 kD; TIP — 80оС; LIV — more than 7 days; DEP — 10-4. In a refrigerator (is not higher 4оС) the infectivity of the phryma sap proceeded not less than 20 days. Against a virus the antiserum is obtained about which titer has made 1/12800. The antigenic affinity with the tobacco ring spot virus is shown. According to these data the pathogen falls belongs to Nepoviruses.

The functional groups diversity of xerophytic common oak forest in natural monument «nizhne-bannovskij» saratov region The article is devoted to the analyses of functional groups of xerophytic common oak forest in natural monument «Nizhne-Bannovskij». A standard distributions of plants species (geometric rows and MacArthur), corresponding to the experimental one, and their comparison with the help of normalized Shannon index were applied. Different groups of stability were established and its ecological structure was performed. Comparison of parameters of ecological structure of xerophytic common oak forest with those forest, steppe communities and ecotones are performed.

The ecological and biological features of Atraphaxis frutescens cenopopulations in left Volga bank of Saratov region The article is devoted to the cenopopulation structure of Atraphaxis frutescens in the south left Volga bank of Saratov region. The patterns of community with this rare species are performed.

Morphogenesis and regeneration of inflorescence axis in culture Iris ensata Thunb. The results of anatomical-morphological regeneration-investigation in explants of inflorescence axis of some cultivars I. ensata Thunb. are presented there. Morphogenesis peculiarities and regenerative activity of the given type of explants are found out. It’s noted, that morphogenesis is carried out similar to gemmagenesis, passing by the stage of callus formation. Shoots, formed de novo, were of endogenous origin.

A comparative analysis of caesium-137 and potassium-40 accumulation in grassy vegetation of the radioactive contaminated territory of Tula region Comparative analysis of 137Cs and 40K accumulation in agrophitocaenosis of field crop rotation (wheat, barley, potatoes) and natural meadows of Plavsk radioactive spot (Tula region) showed that the values of the specific activity of radionuclides are 40–740 Bq/kg and 205–385 Bq/kg respectively. 137Cs accumulates mostly in the roots, and 40K is evenly distributed in plant organs with a somewhat larger fraction of accumulation in aboveground phytomass.

Clinical and immunological peculiarities of whooping cough at children of early age in modern stage The article show clinical and immunological peculiarities of whooping cough at children of early age. Clinical manifestation of modern whooping cough characterized by light symptoms of intoxication, paroxysmal cough, more often at night and finished discharge of viscous sputum or vomiting, sometimes with reprizes; at percussion — box sound, at auscultation — rigid breathing with dry rales. In peripheral blood — leukocytosis, lymphocytosis, accelerated ESR. Increased level of cytokines, decreased level of subpopulations of CD3+ and CD4+ lymphocytes against a background of physiological level of CD8+ and CD19+, decreased level of factors of local immunity.