14.03.00 Медико-биологические науки

Research on the influence of endogenous bioregulators, isolated from blood serum and bones of mammals, on the regeneration of amphibian limbs The purpose of this study was to investigate the effect of bioregulators (BR) on anurans limb regeneration and to maintain the viability of the tissues of the developing salamanders regenerate in vitro. During this work it was found that the ability of Xenopus laevis to regenerate hindlimb in metamorphosis stage depends on the level of amputation, while stimulating regeneration by BR isolated from blood serum, decreases when the regenerative capacity increases. BR from serum had a pronounced effect on the regeneration of Xenopus laevis hindlimb in vivo when amputation was in the proximal region. BR from bone and BR from serum had a protective effect on the condition of the tissue regenerated limb of the newt Pleurodeles waltl provided by cultivation in vitro.  

Investigation of thermal resistance and antagonistic properties of the yeast Saccharomyces cerevisiae Using antagonistic properties of some bacteria to inhibit other (pathogenic) has been studied for many years, while little attention was paid to study such properties in yeast. The yeast metabolites and yeast cells action on gram-negative and gram-positive microorganisms was different. Live yeast cells were the most antagonistically active against gram-positive microorganisms and their metabolites - in relation to gram-negative.  

Regularities of formation and erosion processes during spring snowmelt on the territory of agrolandscape and their impact on the quality of river water Analysis of the results four years of research of the snowmelt runoff formation and soil loss on the catchment area of the small river Lubozhikha showed that surface runoff and soil washout has an influence not only on the intensity of erosion processes, but also on the chemical composition of river waters.  

The search of probiotic culture of Bacillus subtilis on medicinal herbs’ biomass using the phages The culture of Bacillus subtilis witch can include probiotic strains was identified on the biomass of herbs (mint, camomile, milk thistle) using specific bacteriopfage. The authors suggest that medicinal herbs possess useful properties not only because of the biologically active substances, but also due to the content of enhancing therapeutic effect Bacillus subtilis in their decoction.

The study of microbial activity of multicomponent of eye drops for the treatment of conjunctivitis Microbiological activity of model mixtures of eye drops for the treatment of complex action conjunctivitis in cultures opportunistic pathogens St. epidermidis and Candida albicans oftalmomikozov been studied. The sterility of test solutions of eye drops has been studied. The zone destruction of microorganisms has been established.

Determination of the spectrum of lytic activity of bacteriophages by the electro-optical analysis of cell suspensions Changes in the electrooptical (EO) properties of cell suspensions of the Azospirillum brasilense strains Sp7, Cd, Sp107, Br14, KR77, S27, SR55, Sr75, Sp245, Jm6B2, S17, A. lipoferum strains Sp59b, Sr65, RG20am A. amazonense Am14, A. halopraeferans Au4, A. irakense strains KBC1 and KA3 d) in their contamination with the bacteriophage ΦAb-SR75 were studied depending on the infection. Control experiments were carried out to infect the cells with the bacteriophage, by making a standard inoculation of the dense nutrient media. The fact that the method for EO analysis of cell suspensions may be used to determine the spectrum of lytic activity of bacteriophages is first shown.

Strategy and tactics for control of viral diseases of plants for example potatoes The article offers a conceptual approach to the problem of control of viral diseases of plants. It is a selection of key strategic measures, taking into account biology of the host plant and features of viral infection. These include preventive measures related to seed production in virus-free basis, breeding for virus resistance and biotechnological methods of health promotion and propagation of virus-free material. These areas are mainly associated tactics of its receipt.

Broncho-Pulmonary Pathology as Leading Cause of Hospitalization and Death of HIV-Patients in Providing Urgent Medical Care at Multifield Medical Prophylactic Institution The causes of hospitalization and mortality of  HIV-infected patients (n=20) during 10-year period of medical treatment in a multidisciplinary health care setting (Military Medical Academy) were analyzed. Today the main causes of these persons death in these institutions are the diseases not associated with HIV infection: burns, combined injuries, toxic substances poisoning. It was found that HIV infection worsens the prognosis of patients hospitalized with respiratory apparatus diseases, promoting the earlier development of nosocomial infections, exacerbation of  broncho-pulmonary diseases and short-term mortality.  

Phytosanitary situation in a potato crop associated with the spread of viral diseases in the Astrakhan region The article describes the phytosanitary monitoring in a potato crop in the Astrakhan region. The materials obtained have shown that the strict implementation of previously recommended preventive measures to protect the normalized 2011 phytosanitary condition of potato fields with plantings associated with virus and phytoplasma infection in the Astrakhan region. However, in 2012—2014 years again, a difficult situation in terms epiphytotic viral infection.

Assessing the current state of the epidemic spread of HIV infection in the city of Magnitogorsk, Chelyabinsk region The purpose of this study is to evaluate the epidemic situation in the incidence of HIV infection in the city of Magnitogorsk, Chelyabinsk region, to identify the main features and trends in the epidemic. The epidemiology of HIV infection in Magnitogorsk is a high intensity spread of the disease among the population with signs of generalization. Identified the following unfavorable trends in the spread of HIV infection: active involvement in the epidemic of female and age of the population; increase the spread of HIV among drug users and patients with infections, sexually transmitted infections; increase in the number of patients with clinically advanced stages of HIV infection; increase the hidden sources of HIV infection; high mortality rate among patients with HIV infection associated with tuberculosis.

Innovation vaccines against dangerous viral diseases The experience of the development of candidate mucosal plant-made vaccines of oral administration. As an example the data about the development of vaccines against HIV-1/AIDS, hepatitis B and cervical cancer are considered in the paper. Resuls of the induction of immune responses in mice after the vaccination with dried fruits of transgenic tomato plants containing antigenic proteins TBI-HBS, PreS2-S and HPV16 L1 are reported.  

Effects of viral infection on the protein composition of the thylakoid membrane of tomato, pepper and cucumber Cucumber mosaic virus (CMV) and Tomato mosaic virus (TMV) causing viral diseases in many important plants worldwide. Thylakoid membranes were isolated from chloroplasts of infected plants (cucumber, pepper and tomato) and analyzed the protein composition by electrophoresis on PAGE gradient gel (10–25%). The gradient electrophoresis of thylakoid membrane proteins of virus-infected plants was analyzed pepper and tomato plants infected with TMV virus and cucumber plant infected with CMV virus revealed that the content of polypeptide in the 47, 33, 29–24 and 17–15 kDa comprising the submolecular complex of photosystem II complex and oxygen evolving complex significantly reduced compared with uninfected plants that may be associated with structural abnormalities in the complex PS II.

Application of cellular technologies for treatment of viral diseases The review is devoted to a hot topic — application of cellular technologies for treatment of viral diseases, in particular for treatment of AIDS.

Virology Today: Integration of D. I. Ivanovslii’s Ideas into the Methodology of Science In his research on the tobacco mosaic virus D. I. Ivanovskii has introduced a new type of causative agent of an infectious desease and a new form of life. In this way he has layed the basis of a ne field of science, virology. Its structure of knowledge and the logics of development caused formation of new scientific areas that go beyond the scope of natural science. The cognitive model of virology as science has become the prototype of computer virology and has influenced the development of communication theory.

Food product as a source of viral infections The main routes of transmission of viruses that can enter the body through infected food were considered. It has been shown that viruses are resistant to adverse environmental conditions and may be stored for several months in food and environment. Common sources, which can accumulate enteroviruses, Coxsackie B5 virus, hog cholera virus (HCV), African Swine Fever virus (ASFV), FMD virus, poliovirus, rotavirus and echovirus are described.

The influence of vertically transmitted virus infections on the spinal cord injury progression in newborn children The work represents the results of the radiological, clinical and laboratory examinations of 380 newborn children with a personal history of the spinal cord injury. The optimal examination of the newly-born children with birth traumas has been worked out. The pathology of the birth spinal cord injury in children with clinical evidence of vertically transmitted virus infection has been analysed.

Immunopathogenesis and therapy of liver disease of viral etiology Motivated immune pathogenesis of HCV viral infection. The analysis of immune dysfunction in chronic viral hepatitis, and its deepening in the context of the standard therapy, also the possibility of correction of drugs cytokines. The results and prospects of the use of Roncoleukin when optimizing the treatment of patients with chronic viral hepatitis presented.

The epidemiological situation of West Nile fever in Volgograd region West Nile virus for the Volgograd region is the most important arbovirus infection. The observed decrease in the incidence of WNV from 2001 to 2006, in 2008 and 2009 years, reducing the number of severe disease is only indicative of a temporary regression of the epidemic process in the Volgograd region. In the Volgograd region there are prerequisites and precursors complications epidemiology of West Nile fever. The purpose held in the Volgograd region surveillance in West Nile is to provide the information necessary and sufficient for the analysis of the epidemiological situation, as well as to determine the direction of policy and action in public health management decisions.

Molecular detection of baculoviruses of beet webworm from the collection of All-Russian Institute for Plant Protection Two isolates of baculoviruses from beet webworm Loxostege sticticalis (Lepidoptera: Pyraloidea) deposited in the collection of entomopathogenic organisms as a granulovirus (GVLS-85) and a nucleopolyhedrovirus (NPVLS-90) have been subjected to genotyping using degenerate primers L8F2:L8R2 flanking a conservative region of late elongation factor gene lef8. Both isolates, tested independently to avoid sample cross-contamination, were found to belong to a single molecular haplotype. This haplotype is unique, showing maximum sequence similarity of 73% to Pieris rapae granulovirus (Genbank accession number JX968491) and other granuloviruses. No significant similarity to available sequences of nucleopolyhedroviruses have been found.