03.02.02. Вирусология. Подрубрика: математические модели в прогнозировании распространения вирусов

Spreading of virus diseases of fruit-trees and small fruit cultures and modern techniques for their sanitation The spreading of different viruses on varieties and rootstocks of pome fruit trees, stone fruit plants, and small fruit cultures has been studied. It was established that efficiency of sanitation technique from viruses depended on culture, virus species and therapy method. The best results for obtaining virus free material were achieved by means of complex application of in vitro chemotherapy and magnetotherapy.

Preservation of infectious activity of industrial strains of rabies virus stored at different temperatures The dependence of specific infectious activity on the cell culture model of rabies virus industrial (fixed) strains L. Pasteur and CVS from the duration and storage temperature was investigated. Viral suspension was produced by cell culture BHK-21 / C13 and frozen at -20 and -80°C. Analysis of the specific activity was performed after 1 week, 1, 2, 3, 6 and 12 months of storage. It was experimentally discovered that the temperature -80°C is more effective for long-term storage for the studied strains.

Lithium salt of acrydon-acetic acid as a substance for biomedical research The article considers a new antiviral and imunomodulating drug with extremely high bactericidal, and particularly virucidal activity against RNA and DNA viruses, mycocidal and algicidal effect. Ways of the effective use of the drug in the national economic products are discussed.

The hybrid forecasting model on the basis of intellectual agents The hybrid model on the basis of intellectual agents is developed for the task of infectious dynamics prediction. Intellectual agents are created as recurrent neural networks and interact on the basis of the Markov chain. In comparison with traditional mathematical models the hybrid forecasting model of infectious dynamics has following advantages: take into account epidemic’s numerous parameters, possibility of model’s scaling and setup for different types of epidemics.

Тhe frequency of the human papillomavirus in the population of the Rostov-on-Don In this study the detection frequency of human papilloma virus types with high carcinogenic risk among residents of Rostov-on-Don was analyzed. The frequency of HPV-positive samples among women was 40%, among men — 21, 5%. Detection frequency of human papillomavirus was highest among young persons up to 35 years. More than 40% of HPV-positive persons had an average concentration of virus which may cause the cell dysplasia.

Virus inhibitory effect of the complex interferon inducer of type I in vivo in experimental herpes infection Ativiral effect of the molecular composition (MC) yeast RNA-tilorone and 2, 7-bis[2-(diethylamino)ethoxy]-fluoren-9-on dihydrochloride (tilorone) was demonstrated on the experimental model of herpes infection in vivo. The reduction of mortality by 80 % was observed in the group of herpes-infected animals treated with the MC, and the average life expectancy increased by 1, 5 times compared to the control. A reduction of the level of herpes simplex virus replication in the infected animal brain tissue was identified as the result of MC activity. The molecular composition of the yeast RNA and tilorone showed the most significant anti-herpetic effect comparing to the officinal drugs — Virolex and рoly(І)-poly(C).

The founder of the doctrine of viruses (To the 150th anniversary of the birthday of D. I. Ivanovsky) Describes the main stages of scientific and pedagogical activity of the D. I. Ivanovsky. Main results of his research, which determine the undisputed priority of the scientist as the founder of the doctrine of viruses.

Study of the stability of collection of potato varieties to virus diseases in the forest-steppe of Ukraine Studied 630 potato varieties for resistance to the major viral diseases in the forest-steppe of Ukraine. Established that the visual assessment of diseases complicated by nonspecific symptoms of some viral diseases. Using immunological and electron microscopic methods were identified pathogens of viral infections — Potato virus M and Potato virus Y. Highlighted accessions resistant to potato leaf roll, leafdrop streak and wrinkled mosaic.

On a plant Phryma asiatica (Hara) Probat. for the first time is revealed the isometric virus with the following characteristics: diameter virions — 30–32 nanometers; molecular weight of a capsid protein — 41,4 ± 0,8 kD; TIP — 80оС; LIV — more than 7 days; DEP — 10-4. In a refrigerator (is not higher 4оС) the infectivity of the phryma sap proceeded not less than 20 days. Against a virus the antiserum is obtained about which titer has made 1/12800. The antigenic affinity with the tobacco ring spot virus is shown. According to these data the pathogen falls belongs to Nepoviruses.

The search of probiotic culture of Bacillus subtilis on medicinal herbs’ biomass using the phages The culture of Bacillus subtilis witch can include probiotic strains was identified on the biomass of herbs (mint, camomile, milk thistle) using specific bacteriopfage. The authors suggest that medicinal herbs possess useful properties not only because of the biologically active substances, but also due to the content of enhancing therapeutic effect Bacillus subtilis in their decoction.

The study of microbial activity of multicomponent of eye drops for the treatment of conjunctivitis Microbiological activity of model mixtures of eye drops for the treatment of complex action conjunctivitis in cultures opportunistic pathogens St. epidermidis and Candida albicans oftalmomikozov been studied. The sterility of test solutions of eye drops has been studied. The zone destruction of microorganisms has been established.

Determination of the spectrum of lytic activity of bacteriophages by the electro-optical analysis of cell suspensions Changes in the electrooptical (EO) properties of cell suspensions of the Azospirillum brasilense strains Sp7, Cd, Sp107, Br14, KR77, S27, SR55, Sr75, Sp245, Jm6B2, S17, A. lipoferum strains Sp59b, Sr65, RG20am A. amazonense Am14, A. halopraeferans Au4, A. irakense strains KBC1 and KA3 d) in their contamination with the bacteriophage ΦAb-SR75 were studied depending on the infection. Control experiments were carried out to infect the cells with the bacteriophage, by making a standard inoculation of the dense nutrient media. The fact that the method for EO analysis of cell suspensions may be used to determine the spectrum of lytic activity of bacteriophages is first shown.

Strategy and tactics for control of viral diseases of plants for example potatoes The article offers a conceptual approach to the problem of control of viral diseases of plants. It is a selection of key strategic measures, taking into account biology of the host plant and features of viral infection. These include preventive measures related to seed production in virus-free basis, breeding for virus resistance and biotechnological methods of health promotion and propagation of virus-free material. These areas are mainly associated tactics of its receipt.

Broncho-Pulmonary Pathology as Leading Cause of Hospitalization and Death of HIV-Patients in Providing Urgent Medical Care at Multifield Medical Prophylactic Institution The causes of hospitalization and mortality of  HIV-infected patients (n=20) during 10-year period of medical treatment in a multidisciplinary health care setting (Military Medical Academy) were analyzed. Today the main causes of these persons death in these institutions are the diseases not associated with HIV infection: burns, combined injuries, toxic substances poisoning. It was found that HIV infection worsens the prognosis of patients hospitalized with respiratory apparatus diseases, promoting the earlier development of nosocomial infections, exacerbation of  broncho-pulmonary diseases and short-term mortality.  

Phytosanitary situation in a potato crop associated with the spread of viral diseases in the Astrakhan region The article describes the phytosanitary monitoring in a potato crop in the Astrakhan region. The materials obtained have shown that the strict implementation of previously recommended preventive measures to protect the normalized 2011 phytosanitary condition of potato fields with plantings associated with virus and phytoplasma infection in the Astrakhan region. However, in 2012—2014 years again, a difficult situation in terms epiphytotic viral infection.

Assessing the current state of the epidemic spread of HIV infection in the city of Magnitogorsk, Chelyabinsk region The purpose of this study is to evaluate the epidemic situation in the incidence of HIV infection in the city of Magnitogorsk, Chelyabinsk region, to identify the main features and trends in the epidemic. The epidemiology of HIV infection in Magnitogorsk is a high intensity spread of the disease among the population with signs of generalization. Identified the following unfavorable trends in the spread of HIV infection: active involvement in the epidemic of female and age of the population; increase the spread of HIV among drug users and patients with infections, sexually transmitted infections; increase in the number of patients with clinically advanced stages of HIV infection; increase the hidden sources of HIV infection; high mortality rate among patients with HIV infection associated with tuberculosis.

Innovation vaccines against dangerous viral diseases The experience of the development of candidate mucosal plant-made vaccines of oral administration. As an example the data about the development of vaccines against HIV-1/AIDS, hepatitis B and cervical cancer are considered in the paper. Resuls of the induction of immune responses in mice after the vaccination with dried fruits of transgenic tomato plants containing antigenic proteins TBI-HBS, PreS2-S and HPV16 L1 are reported.  

Effects of viral infection on the protein composition of the thylakoid membrane of tomato, pepper and cucumber Cucumber mosaic virus (CMV) and Tomato mosaic virus (TMV) causing viral diseases in many important plants worldwide. Thylakoid membranes were isolated from chloroplasts of infected plants (cucumber, pepper and tomato) and analyzed the protein composition by electrophoresis on PAGE gradient gel (10–25%). The gradient electrophoresis of thylakoid membrane proteins of virus-infected plants was analyzed pepper and tomato plants infected with TMV virus and cucumber plant infected with CMV virus revealed that the content of polypeptide in the 47, 33, 29–24 and 17–15 kDa comprising the submolecular complex of photosystem II complex and oxygen evolving complex significantly reduced compared with uninfected plants that may be associated with structural abnormalities in the complex PS II.

Application of cellular technologies for treatment of viral diseases The review is devoted to a hot topic — application of cellular technologies for treatment of viral diseases, in particular for treatment of AIDS.

Virology Today: Integration of D. I. Ivanovslii’s Ideas into the Methodology of Science In his research on the tobacco mosaic virus D. I. Ivanovskii has introduced a new type of causative agent of an infectious desease and a new form of life. In this way he has layed the basis of a ne field of science, virology. Its structure of knowledge and the logics of development caused formation of new scientific areas that go beyond the scope of natural science. The cognitive model of virology as science has become the prototype of computer virology and has influenced the development of communication theory.