03.01.06 Биотехнология (в том числе бионанотехнологии)

Orthobuniaviruses as main factor for emergency diseases This analytical paper deals with the systematic and taxonomy of orthobuniaviruses, small developed dangerous and new viruses Akabane, Aino, Schmallenberg, Cache Valley diseases, Oropouche fever, Iquitos. The significance of reassortment mechanism of their origin and diversification are discussed.

Spreading of virus diseases of fruit-trees and small fruit cultures and modern techniques for their sanitation The spreading of different viruses on varieties and rootstocks of pome fruit trees, stone fruit plants, and small fruit cultures has been studied. It was established that efficiency of sanitation technique from viruses depended on culture, virus species and therapy method. The best results for obtaining virus free material were achieved by means of complex application of in vitro chemotherapy and magnetotherapy.

Preservation of infectious activity of industrial strains of rabies virus stored at different temperatures The dependence of specific infectious activity on the cell culture model of rabies virus industrial (fixed) strains L. Pasteur and CVS from the duration and storage temperature was investigated. Viral suspension was produced by cell culture BHK-21 / C13 and frozen at -20 and -80°C. Analysis of the specific activity was performed after 1 week, 1, 2, 3, 6 and 12 months of storage. It was experimentally discovered that the temperature -80°C is more effective for long-term storage for the studied strains.

Lithium salt of acrydon-acetic acid as a substance for biomedical research The article considers a new antiviral and imunomodulating drug with extremely high bactericidal, and particularly virucidal activity against RNA and DNA viruses, mycocidal and algicidal effect. Ways of the effective use of the drug in the national economic products are discussed.

The hybrid forecasting model on the basis of intellectual agents The hybrid model on the basis of intellectual agents is developed for the task of infectious dynamics prediction. Intellectual agents are created as recurrent neural networks and interact on the basis of the Markov chain. In comparison with traditional mathematical models the hybrid forecasting model of infectious dynamics has following advantages: take into account epidemic’s numerous parameters, possibility of model’s scaling and setup for different types of epidemics.

Тhe frequency of the human papillomavirus in the population of the Rostov-on-Don In this study the detection frequency of human papilloma virus types with high carcinogenic risk among residents of Rostov-on-Don was analyzed. The frequency of HPV-positive samples among women was 40%, among men — 21, 5%. Detection frequency of human papillomavirus was highest among young persons up to 35 years. More than 40% of HPV-positive persons had an average concentration of virus which may cause the cell dysplasia.

Virus inhibitory effect of the complex interferon inducer of type I in vivo in experimental herpes infection Ativiral effect of the molecular composition (MC) yeast RNA-tilorone and 2, 7-bis[2-(diethylamino)ethoxy]-fluoren-9-on dihydrochloride (tilorone) was demonstrated on the experimental model of herpes infection in vivo. The reduction of mortality by 80 % was observed in the group of herpes-infected animals treated with the MC, and the average life expectancy increased by 1, 5 times compared to the control. A reduction of the level of herpes simplex virus replication in the infected animal brain tissue was identified as the result of MC activity. The molecular composition of the yeast RNA and tilorone showed the most significant anti-herpetic effect comparing to the officinal drugs — Virolex and рoly(І)-poly(C).

The founder of the doctrine of viruses (To the 150th anniversary of the birthday of D. I. Ivanovsky) Describes the main stages of scientific and pedagogical activity of the D. I. Ivanovsky. Main results of his research, which determine the undisputed priority of the scientist as the founder of the doctrine of viruses.

Study of the stability of collection of potato varieties to virus diseases in the forest-steppe of Ukraine Studied 630 potato varieties for resistance to the major viral diseases in the forest-steppe of Ukraine. Established that the visual assessment of diseases complicated by nonspecific symptoms of some viral diseases. Using immunological and electron microscopic methods were identified pathogens of viral infections — Potato virus M and Potato virus Y. Highlighted accessions resistant to potato leaf roll, leafdrop streak and wrinkled mosaic.

On a plant Phryma asiatica (Hara) Probat. for the first time is revealed the isometric virus with the following characteristics: diameter virions — 30–32 nanometers; molecular weight of a capsid protein — 41,4 ± 0,8 kD; TIP — 80оС; LIV — more than 7 days; DEP — 10-4. In a refrigerator (is not higher 4оС) the infectivity of the phryma sap proceeded not less than 20 days. Against a virus the antiserum is obtained about which titer has made 1/12800. The antigenic affinity with the tobacco ring spot virus is shown. According to these data the pathogen falls belongs to Nepoviruses.

The accumulation of toxicants in the body of bees and honey in the conditions of ecologically crisis areas of the Republic of Tatarstan The article is devoted to the study of contamination of honey bees and honey toxicants in oil-producing regions of the Republic of Tatarstan. The results of the accumulation of a number of toxicants in the body of bees and honey.

Monitoring of mushrooms Agaricus bisporus (J. Lge) Imbach: diagnostics and prevention of diseases We conducted research and experiments and we determined that the mushrooms (Agaricus bisporus (J. Lge) Imbach) infected with bacteria, viruses, microscopic fungi. We recommend prevention activities to fight infections.

The comparison of standard metabolic rate and respiratory quotient in Eurasian blue tit (Cyanistes caeruleus, Aves) during winter and summer Standard metabolic rate in the Eurasian blue tit during winter significantly lower than during summer. Respiratory quotient in the Eurasian blue tit during winter higher than during summer, but not significantly. Standard metabolic rate and respiratory quotient had well pronounced diurnal rhythms with minimum in the nighttime (from 2 a. m. to 5 a. m.) and maximum in the daytime. The maximum difference between standard metabolic rates during daytime and nighttime was 45 % during winter and 15 % during summer.

The effect of foliar feeding of micronutrients on the formation yield of spring rape seeds One of the promising directions in improvement of technology of cultivation of spring oilseed rape is the application of microelements. The results of the studies have revealed increasing the yield of spring rape seeds by 19.7 % with foliar feeding is examined boric and sulfur fertilizers.

The study of the allelic state of microsatellite loci of sugar beet (Beta vulgaris L.) The results of studies of the genetic polymorphism in varieties and hybrids of sugar beet, of which several were polymorphic, are presented. Five types of alleles at marker 086 GZM were identified and analyzed using SSR-analysis. Established genetic similarity between two pairs of hybrids through the test locus, may indicate their common origin.

Bayesian analysis of 2?2 contingency tables relative proportions A new standard expression for distribution of epidemiological risk ratio estimates has been found out using two by two methods of study. Comparing homogeneous groups the distribution is operational within wide-ranging conditions studying both rare and frequent events. Support value of the distribution formula will increase primarily in cases with small amount of the cases or controls observed. Using the derived distribution excess mortality from lung cancer of the Mayak PA workers was associated with radiation exposure with high reliability of the observation.

The comparative study of the influence of pathogenic factors on the formation of congenital and acquired neurosensory hearing loss The aim is to carry out a comparative analysis of pathogenic factors influence on the formation of congenital and acquired NHL and on the formation of complex pathologies and peculiarities of psycho-physiological status of the child. Сhildren with NHL have a physical developmental lag in the process of individual ontogenesis, which is manifested in the lack of functional maturity of somatic and visceral systems of the body, the simultaneous presence of different of anomalies of growth and development. The comparative analysis of the influence of the environmental pathogenic factors on the formation of the congenital and the acquired NHL showed that the condition of intra- and inter-hemispheric integration and the nature of spectral power density of the EEG ranges in children with NHL can act as a functional criterion for the pathology development.

Problems and prospects of the main biogenic elements intramolecular isotope correlations using as a new resource of different aetiology pathologies diagnostics It is shown that chemical elements isotopes fractioning in chemical and biochemical reactions is based on two fundamental kernel properties — the mass and the spin; differences between them can affect on both chemical reactions speed and reactive systems energy state. In some cases deviations in mass-dependent linear isotopic effect for most of uneven isotopes from hydrogen to uranium are observed. These fractioning peculiarities can be used: in living organisms — for early disease detection of different aetiology pathology; in abiogenous natural systems — for new methods of heavy elements isotopes enrichment development.

Formation of the liver in amphibian larval period on the example of the green toad (Bufo Viridis Laurenti, 1768) Describes the morpho-physiological characteristics of the emerging liver in larvae of the green toad (Bufo viridis Laurenti, 1768). Identified that studied the body performs the function of blood during larval development period. In the liver are formed elements of the blood of all lines. Describes he specifics of the organization and morphological changes of the body from developing larvae. There are some pathological changes in the liver and formed elements of blood.

Kasatik mistrue (Iris notha Bieb.) in Azov district of Rostov Province Results of the two Iris notha Bieb. cenopopulations studies in Azov district of Rostov Province in a valley of Vodinskaja gorge (river Tchumburka bassin), first registered in 2014 are presented. There are determined: population area, quantity, density, age structure of cenopopulations, seeds productivity, limiting factors.